Plant Functional GenomicsAs more and more plant genomes are sequenced, plant researchers have been inundated with an avalanche of novel methodologies to identify the function of tens of thousands of plant genes. In Plant Functional Genomics, Erich Grotewold has assembled a team of leading plant scientists to describe in detail the most commonly used methods for investigating plant gene function in a wide variety of plants, during plant pathogen interactions, and even in algae. These readily reproducible protocols include computational, molecular, and genetic methodologies designed for both general and specific problems. Here the reader will learn how to identify genes in complex systems that have large genomes, few cells, and mixed cell systems. Readers will also learn the use of powerful computational and statistical tools to help predict gene function, either on the basis of comparative genomics, or from the analysis of complex genome sequences. Because establishing gene function relies on the identification of phenotypes, the authors expand the concept of phenotypes, including the use of multiple outputs as the ultimate phenotypic result of changes in gene activity. Numerous loss-of-function and gain-of-function techniques for discovering gene function are presented in step-by-step detail. Comprehensive and highly practical, Plant Functional Genomics offers plant biologists readily reproducible computational, molecular, and genetic protocols, powerful tools that will enable them, with little or no experience, successfully to investigate any gene function with the most recent methodologies. |
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Inhalt
An Improved Method for Plant BAC Library Construction | 3 |
Constructing GeneEnriched Plant Genomic Libraries Using Methylation Filtration Technology | 21 |
RescueMu Protocols for Maize Functional Genomics | 37 |
Precious Cells Contain Precious Information Strategies and Pitfalls in Expression Analysis from a Few Cells | 59 |
Combined ESTs from PlantMicrobe Interactions Using GC Counting to Determine the Species of Origin | 79 |
IN SILICO PREDICTION OF PLANT GENE FUNCTION | 85 |
Computer Software to Find Genes in Plant Genomic DNA | 87 |
Genomic Colinearity as a Tool for Plant Gene Isolation | 109 |
HighThroughput TAILPCR as a Tool to Identify DNA Flanking Insertions | 241 |
Custom KnockOuts with Hairpin RNAMediated Gene Silencing | 273 |
VirusInduced Gene Silencing | 287 |
Exploring the Potential of Plant RNase P as a Functional Genomics Tool | 295 |
Maintaining Collections of Mutants for Plant Functional Genomics | 311 |
GAIN OF FUNCTION APPROACHES | 327 |
Vector Construction for Gene Overexpression as a Tool to Elucidate Gene Function | 329 |
TDNA Activation Tagging | 345 |
Using Natural Allelic Diversity to Evaluate Gene Function | 123 |
Quantitative Trait Locus Analysis as a Gene Discovery Tool | 141 |
FORWARD AND REVERSE GENETIC STRATEGIES | 155 |
Transposon Tagging Using Activator Ac in Maize | 157 |
TDNA Mutagenesis in Arabidopsis | 177 |
Physical and Chemical Mutagenesis | 189 |
HighThroughput TILLING for Functional Genomics | 205 |
Gene and Enhancer Traps for Gene Discovery | 221 |
PHENOTYPIC PROFILING AS A TOOL TO DETERMINE GENE FUNCTION | 363 |
Expression Profiling Using cDNA Microarrays | 365 |
Open Architecture Expression Profiling of Plant Transcriptomes and Gene Discovery Using GeneCalling Technology | 381 |
Proteomics as a Functional Genomics Tool | 395 |
Metabolite Profiling as a Functional Genomics Tool | 415 |
Growth StageBased Phenotypic Profiling of Plants | 427 |
| 443 | |
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Acad acid activation tagging agarose gel Agrobacterium allele amplify analysis approx Arabidopsis thaliana Biol buffer cDNA cDNA fragments centrifuge chromosome cloning coli column containing cycles database detected digestion dilution electroporation ethanol exons flanking Functional Genomics gene expression gene prediction GeneCalling GeneMark.hmm genetic genomic DNA genomic sequence Genscan growth stage high-throughput HT-TAIL-PCR hybridization identify Incubate insertion Intr Invitrogen kanamycin laboratory ligation lines maize mapping markers Methods and Protocols microarray mixture Molecular mRNA mutagen mutagenesis mutation Mzef Natl Note nucleotide overexpression PCR product pellet phenotype Plant Cell Plant Functional Genomics plasmid plate polymerase population primer probes protein Qiagen reaction regions RescueMu restriction enzyme Resuspend rice RNase room temperature samples screen seeds selection solution specific step Subheading T-DNA TAIL-PCR target mRNA TBE buffer thermal cycler tion tissue trait transcription transformation transgenic transposable transposon Tris-HCl tube vector wash
Bibliografische Informationen
| Titel | Plant Functional Genomics Band 236 von Methods in molecular biology |
| Herausgeber | Erich Grotewold |
| Verlag | Springer Science & Business Media, 2003 |
| ISBN | 1592594131, 9781592594139 |
| Länge | 449 Seiten |
| Zitat exportieren | BiBTeX EndNote RefMan |