Plant Tissue Culture: Techniques and Experiments

Front Cover
Robert H. Smith
Academic Press, Dec 2, 2012 - Science - 190 pages
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Plant Tissue Culture Techniques and Experiments is a manual that contains laboratory exercises about the demonstration of the methods and different plant materials used in plant tissue culture. It provides an overview on the plant cell culture techniques and plant material options in selecting the explant source.
This book starts by discussing the proper setup of a tissue culture laboratory and the selection of the culture medium. It then explains the determination of an explant which is the ultimate goal of the cell culture project. The explant is a piece of plant tissue that is used in tissue culture. Furthermore, the book discusses topics about callus induction, regeneration and morphogenesis process, and haploid plants from anther and pollen culture. The meristem culture for virus-free plants and in vitro propagation for commercial propagation of ornamentals are also explained in this manual. The book also provides topics and exercises on the protoplast isolation and fusion and agrobacterium-mediated transformation of plants.
This manual is intended for college students, both graduate and undergraduate, who study chemistry, plant anatomy, and plant physiology.
 

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Contents

Chapter 1 Setup of a Tissue Culture Laboratory
1
Chapter 2 Media Components and Preparation
7
Chapter 3 Explant Preparation
19
Chapter 4 Callus Induction
29
Chapter 5 Regeneration and Morphogenesis
53
Chapter 6 Haploid Plants from Anther Culture
69
Chapter 7 Embryo Rescue
81
Chapter 8 Meristem Culture for VirusFree Plants
89
Chapter 10 Protoplast Isolation and Fusion
127
Chapter 11 AgrobacteriumMediated Transformation of Plants
133
Chapter 12 Useful Measurements
147
Chapter 13 Review of Solution Preparation
149
Chapter 14 List of Suppliers
153
Chapter 15 Common Plant Tissue Culture Terms
155
Index
167
Copyright

Chapter 9 In Vitro Propagation for Commercial Production of Ornamentals
99

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