Plant Tissue Culture: Techniques and Experiments
Robert H. Smith
Academic Press, Dec 2, 2012 - Science - 190 pages
Plant Tissue Culture Techniques and Experiments is a manual that contains laboratory exercises about the demonstration of the methods and different plant materials used in plant tissue culture. It provides an overview on the plant cell culture techniques and plant material options in selecting the explant source.
This book starts by discussing the proper setup of a tissue culture laboratory and the selection of the culture medium. It then explains the determination of an explant which is the ultimate goal of the cell culture project. The explant is a piece of plant tissue that is used in tissue culture. Furthermore, the book discusses topics about callus induction, regeneration and morphogenesis process, and haploid plants from anther and pollen culture. The meristem culture for virus-free plants and in vitro propagation for commercial propagation of ornamentals are also explained in this manual. The book also provides topics and exercises on the protoplast isolation and fusion and agrobacterium-mediated transformation of plants.
This manual is intended for college students, both graduate and undergraduate, who study chemistry, plant anatomy, and plant physiology.
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Chapter 2 Media Components and Preparation
Chapter 3 Explant Preparation
Chapter 4 Callus Induction
Chapter 5 Regeneration and Morphogenesis
Chapter 6 Haploid Plants from Anther Culture
Chapter 7 Embryo Rescue
Chapter 8 Meristem Culture for VirusFree Plants
Chapter 9 In Vitro Propagation for Commercial Production of Ornamentals
Other editions - View all
2000-ml Erlenmeyer flask 30 g sucrose acid Add 8 g Adjust pH Adjust volume African violet Agrobacterium anther anther culture Autoclave Autoclave for 15 auxin callus callus induction carbenicillin carrot cell culture chlorine bleach container cul cultivars culture shelf culture tube 25 Cytokinins distilled water Distribute 25 ml embryogenesis embryos EXPLANT PREPARATION flask pour 500 g TC agar genes haploid HortScience IAA stock ized Journal of Botany kinetin Leaf Disk lides liter equivalent mannitol MEDIUM PREPARATION meristem ml myo-inositol stock ml of deion ml per culture ml thiamine stock Morphogenesis Murashige and Skoog myo-inositol NaFeEDTA nitrates petri dish plant cell Plant Physiology Plant propagation plant tissue culture plastic petri dish protoplasts regeneration rinse three root seedling seeds shoot apex Skoog salts Stage sterile plastic petri stock 40 mg/liter subculture sucrose sulfates thiamine thiamine stock 40 tion transfer hood ture vitro vitro propagation volume to 1000