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Two Primary Analytical Measurements
Three Amino Acid Analysis
Four The Dinitrophenyl End Group Method
4 other sections not shown
absorptiometer absorption acetone added alkaline amino acids ammonia anthrone approximately bath Biochem biol blank boiling bone calibration carbohydrate cell cent centrifugation Chem chondroitin sulphate chromatogram chromatography citrate buffer collagen colour concentration connective tissue containing convenient cooled deionisation demineralised dentine described determination dialysis diameter dilute dissolved distilled water DNP-amino acid dried Eastoe elastin elution enzyme ethanol filter flask fractions funnel galactosamine gelatin gelatin solution glass glucosamine glycine heating hexosamine hydrochloric acid hydrolysate hydroxyproline ion-exchange isoionic point layer liquid measured mixed mixture moisture molecular weight molecules mucoprotein N-sodium hydroxide solution ninhydrin obtained optical density ossein particles peak pink portions powder precipitate prepared procedure protein protein solution quantities reaction removed residue resin column reticulin room temperature separation serine sodium sodium hydroxide soluble collagen solvent standard stopper sugars suitable sulphuric acid supernatant test tube tion tyrosine values viscometer viscosity volume washed wavelength