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Introduction and basic equipment
Maintenance of pure culture
Direct observation of microorganisms
11 other sections not shown
1-ml pipettes acid and gas agar surface ampoule anaerobic aqueous aseptically autoclaving autotrophic bacteria boiling broth broth culture bunsen flame butylene glycol cent chemical coagulase coliform colonies colour concentration containing cool cotton wool cover slip crystal violet cytoplasm diameter dilution disc disinfectant Endospores enzymes Escherichia coli examine fermentation fifteen minutes filter paper flagella Flame the mouth flask g Sodium Genus glucose Gram stain grow growth heat incubated Inoculate inverted Label liquid loop MacConkey's broth medium Method methylene blue micro micro-organisms microbiology microscope milk motile nitrate nutrient agar nutrient broth obtained commercially oil-immersion objective oxidation oxygen pathogenic peptone petri dish phenol pigment Place plug positive preparation presence Pseudomonas aeruginosa Requirements Results Ringer's solution rods Salmonella sample slide smear Sodium chloride soil species spirochaetes spores Staphylococcus Staphylococcus aureus steam sterile sterilize by autoclaving straight wire streptococci subculture sulphate tap water technique test tube transfer Wash waterbath yeast