Primary and Stem Cells: Gene Transfer Technologies and Applications
This book describes basic cell engineering methods, emphasizing stem cell applications, and use of the genetically modified stem cells in cell therapy and drug discovery. Together, the chapters introduce and offer insights on new techniques for engineering of stem cells and the delivery of transgenes into stem cells via various viral and non-viral systems. The book offers a guide to the types of manipulations currently available to create genetically engineered stem cells that suit any investigator's purpose, whether it's basic science investigation, creation of disease models and screens, or cells for therapeutic applications.
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AAV vectors adeno-associated virus applications assay BacMam particles baculovirus Biol Biotechnol buffer cardiomyocytes cell lines cell types cellular chromosomes cloning colonies culture differentiation diluted drug discovery electroporation embryonic stem cells enzyme episomal reprogramming episomal vectors Epstein–Barr virus fibroblasts Figure FLIPR fluorescent fragments gene delivery gene expression gene of interest Gene Ther gene therapy gene transfer genetic genome homologous recombination host cell hPSC human cells human embryonic stem human pluripotent stem incubation induced pluripotent stem infection inserted integrase integration iPSCs Klf4 lentiviral lentiviral vectors mammalian cells membrane method modified mouse nuclear nucleofection Oct4 optimization peptides plasmid plates pluripotent stem cells Primary and Stem protein protein transduction protocols reagents replication reprogramming factors retroviral screening sequence SeV vectors somatic cells Sox2 specific stable Technologies transcription transduction transduction efficiency transfection transgene transgene expression transposase transposon viral vectors Virol vitro vivo