Primary and Stem Cells: Gene Transfer Technologies and Applications

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John Wiley & Sons, Oct 31, 2011 - Science - 296 pages
This book describes basic cell engineering methods, emphasizing stem cell applications, and use of the genetically modified stem cells in cell therapy and drug discovery. Together, the chapters introduce and offer insights on new techniques for engineering of stem cells and the delivery of transgenes into stem cells via various viral and non-viral systems. The book offers a guide to the types of manipulations currently available to create genetically engineered stem cells that suit any investigator's purpose, whether it's basic science investigation, creation of disease models and screens, or cells for therapeutic applications.
 

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Contents

DNA Assembly Technologies Based on Homologous
12
Strategies for the Delivery of Naked DNA
37
Episomal Vectors
51
Nonintegrating DNA Virus
85
Nonintegrating RNA Viruses
103
Protein Delivery
119
Sleeping Beauty TransposonMediated Stable Gene
137
Integrating Viral Vectors for Gene Modifications
163
Bacteriophage Integrases for SiteSpecific Integration
199
Improving Gene Targeting Efficiency in Human
211
Modified Stem Cells as Disease Models and in Toxicology
229
Screening and Drug Discovery
251
INDEX
273
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About the author (2011)

UMA LAKSHMIPATHY is a principal investigator at Life Technologies. She has a PhD in life sciences, with academic and industry experience in molecular biology and stem cells. Dr. Lakshmipathy holds four patents and has authored more than forty publications.

BHASKAR THYAGARAJAN is a program manager at Life Technologies. He has a PhD in pharmacology, with expertise in the areas of molecular biology, DNA recombination, gene and cell therapy, and protein purification. He holds one patent and has authored more than twenty publications.

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