Principles and Reactions of Protein Extraction, Purification, and Characterization
Principles and Reactions of Protein Extraction, Purification, and Characterization provides the mechanisms and experimental procedures for classic to cutting-edge techniques used in protein extraction, purification, and characterization.
The author presents the principles and reactions behind each procedure and uses tables to compare the different methods. The book also discusses the development of antibodies and immunochemical techniques as tools for characterizing proteins and modified proteins such as glycoproteins. Helpful illustrations, diagrams, and tables effectively transform theoretical concepts into practical knowledge. Along with methodical working procedures for most techniques, the book also offers useful advice on which technique to use and when to apply a particular method.
Presenting the advantages and disadvantages of the various protein techniques, Principles and Reactions of Protein Extraction, Purification, and Characterization enables students and researchers to master the mechanisms behind the protocols and choose the best method for their purposes.
What people are saying - Write a review
We haven't found any reviews in the usual places.
Chapter 2 Estimation of Protein
Chapter 3 Electrophoretic Analyses of Protein
Chapter 4 Purification of Protein
Structures Interactions and Production
Other editions - View all
acetic acrylamide activity adjuvant alkaline phosphatase amino acid ammonium persulfate Anal antigen antigen-antibody binding Bio-Rad Biochem biotin biotinylated blot blue carbohydrate cells Centrifuge column complex concentration conjugate containing Coomassie cross-linker denaturing destaining detection detergent diluted distilled water electrophoresis ELISA enzyme equilibrating buffer extraction Figure fluorescent fractionation gel filtration glycans glycoproteins gradient gram groups hapten hydrophobic immobilized immune immunoassays immunogenic Incubate interactions ion-exchange isoelectric focusing labeled lectins ligand lysis matrix membrane proteins methanol method mg/ml mixture molecular weight molecules monoclonal antibodies monosaccharides N-hydroxysuccinimide NHS-ester oligosaccharides oxidation peptides peroxidase phosphate plate precipitate Prepare primary amines primary antibody protein assay protein bands protein sample purification reaction reacts remove room temperature SDS-PAGE secondary antibody sensitive separating gel Sepharose sodium soluble stacking gel staining solution stock solution substrate sulfate sulfhydryl supernatant tissue Tris-HCl tube unknown sample usually volume Wash µg/ml