Procedures for the isolation and identification of mycobacteria
Dept. of Health, Education, and Welfare, Public Health Service, Center for Disease Control, Bureau of Laboratories, Training and Consultation Division, 1977 - Medical - 136 pages
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1000 mcg/ml solution 9 ml sterile acid acid-fast bacilli Add 1 ml agar Amer Arylsulfatase Autoclave avium complex biological safety cabinet bovis buffer catalase catalase activity chloride chromogenic clinical colony forms container Cycloserine decontamination dilution discard Dissolve distilled water Drug Medium drug susceptibility test Ethambutol Ethionamide Figure flask flavescens fluid fortuitum gastri gastric lavage growth identification Incubate Inoculate inoculum Isoniazid Kanamycin kansasii Kubica laboratory liquid culture loop Lowenstein MacConkey marinum microscope Middlebrook ml distilled water ml of medium ml sterile distilled mycobacteria negative niacin nonchromogenic nonphotochromogens organisms phenol phosphate photochromogenic pigment pipette plates Preparation PROCEDURE Pyrazinamidase quadrant rapid growers Reagent refrigerator resistant Resp Rifampin room temperature rough saline screw-cap test tubes screw-cap tubes scrofulaceum sediment slide slow growers smear smooth sodium species sputum sterile distilled water strains substrate sulfate szulgai tellurite tuberculosis Tween hydrolysis Uninoculated urease Viomycin weeks xenopi yellow