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Noissmsia cnv sims3u
B SaHH13W JMV S1VIU31VW
Preparation of Lforms
2 other sections not shown
24-hr culture able to produce aerated aerobically agar plates amounts of enterotoxin antibiotic antibiotic plates BHI and BHI-S BHI-S broths catalase cell wall cells/ml check for reversion colonies concentration cultures of L-forms decreased denotes 3-3 Dienes DM agar DM broth DM flasks DM plate due to dialysis EHI-S entero enterotoxin production enterotoxin was detectable enzyme five reverts gram negative Gram stains grown growth in BHI growth occurred incubated inoculum isolated Klieneberger L-colonies L-form growth L-forms derived L-phase variants lyophilized MaCl mannitol media containing medium methicillin method microslides milliliter moniliformis NaCl NAK-PHP normal cells observed parent strains pathogenic pH increase placed in BHI plate counts polyethylene glycol prepared L-forms produce detectable amounts produced enterotoxin producing strains quantities of enterotoxin reaction salt spheroplasts stable L-forms Staphylococcus aureus stationary cultures sucrose supernate temperature test for reversion toxin production transferred to BHI tube of DM tubes of BHI unstable