Protein Engineering For Industrial Biotechnology

Front Cover
Lilia Alberghina
CRC Press, Sep 2, 2003 - Medical - 388 pages
1 Review
Protein engineering has proved to be one of the more fruitful technological approaches in biotechnology, being both very powerful and able to generate valuable intellectual property. This book aims to present examples in which the application of protein engineering has successfully solved problems arising in industrial biotechnology. There is a section on its use to enhance purification of recombinant proteins. The use of protein engineering to modify the activity or the stability of industrial enzymes from lipases to proteases, from carboxypeptidases to glucanases and glucosidases, and from pectin modifying enzymes to enzymes able to degrade recalcitrant compounds is extensively covered. It is shown how areas as diverse as agrofood technology, fine chemistry, detergents, bioremediation and biosensors receive significant contributions from protein and solvent engineering.

The application of protein engineering to health care is also covered, from the development of new vaccines to new potential therapeutic proteins. A specific notation is given to protein engineering in the development of target molecules for drug discovery.

International in scope, the many contributions are drawn from academia and industry. The text should be of interest to students and researchers in industrial biotechnology as well as to everybody interested in basic research in protein structure, molecular genetics, bio-organic chemistry, biochemistry, agrobiotechnology, pharmaceutical sciences and medicine.
 

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Contents

A REVIEW
1
2 ENHANCED RECOVERY AND FOLDING OFRECOMBINANT PROTEINS USING FUSIONPROTEIN STRATEGIES
22
THE STREPTAG
39
FROM MOLECULAREVOLUTION ANALYSIS TO THE DESIGN OF ASYNTHETIC GENE
59
5 STRUCTUREFUNCTION STUDIES ONCUTINASE A SMALL LIPOLYTIC ENZYME WITHA WATER ACCESSIBLE ACTIVE SITE
71
GENETICS STRUCTURE AND APPLICATIONS
110
7 SOLVENT ENGINEERING MODULATESSTEREOSELECTIVITY OF MICROBIAL LIPASES
130
8 STABILIZATION OF INDUSTRIAL ENZYMES BYPROTEIN ENGINEERING
142
13 ENGINEERING PROTEINS FOR THEDEGRADATION OF RECALCITRANT COMPOUNDS
219
14 PROTEIN ENGINEERING FOR BIOSENSORS
237
15 PROTEIN ENGINEERING IN VACCINEDEVELOPMENT
258
16 EXPLORING MOLECULAR RECOGNITION BYCOMBINATORIAL AND RATIONAL APPROACHES
267
17 PRODUCTION AND POTENTIALAPPLICATIONS OF RECOMBINANT GASTRICLIPASES IN BIOTECHNOLOGY
282
PROTEIN ENGINEERING OF A RASSPECIFICGUANINE
297
19 EXPRESSION AND USE OF TAGGED CELLSURFACE RECEPTORS FOR DRUG DISCOVERY
310
20 ESTROGEN RECEPTOR AS A TARGET FORNEW DRUG DISCOVERY
325

9 DESIGN OF BIOTECHNOLOGICALLYAPPLICABLE YEAST CARBOXYPEPTIDASE YMUTANTS WITH INCREASED PREFERENCE FORC...
153
10 ENGINEERINGbGLYCOSIDE HYDROLASES
162
11 ENGINEERING SPECIFICITY AND STABILITY INGLUCOAMYLASE FROMASPERGILLUS NIGER
182
12 PECTIN MODIFYING ENZYMES ANDDESIGNER PECTINS
200
21 MOLECULAR BIOSCREENING IN ONCOLOGY
341
INDEX
354
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