Protein Engineering For Industrial Biotechnology
CRC Press, Sep 2, 2003 - Medical - 388 pages
Protein engineering has proved to be one of the more fruitful technological approaches in biotechnology, being both very powerful and able to generate valuable intellectual property. This book aims to present examples in which the application of protein engineering has successfully solved problems arising in industrial biotechnology. There is a section on its use to enhance purification of recombinant proteins. The use of protein engineering to modify the activity or the stability of industrial enzymes from lipases to proteases, from carboxypeptidases to glucanases and glucosidases, and from pectin modifying enzymes to enzymes able to degrade recalcitrant compounds is extensively covered. It is shown how areas as diverse as agrofood technology, fine chemistry, detergents, bioremediation and biosensors receive significant contributions from protein and solvent engineering.
The application of protein engineering to health care is also covered, from the development of new vaccines to new potential therapeutic proteins. A specific notation is given to protein engineering in the development of target molecules for drug discovery.
International in scope, the many contributions are drawn from academia and industry. The text should be of interest to students and researchers in industrial biotechnology as well as to everybody interested in basic research in protein structure, molecular genetics, bio-organic chemistry, biochemistry, agrobiotechnology, pharmaceutical sciences and medicine.
2 ENHANCED RECOVERY AND FOLDING OFRECOMBINANT PROTEINS USING FUSIONPROTEIN STRATEGIES
FROM MOLECULAREVOLUTION ANALYSIS TO THE DESIGN OF ASYNTHETIC GENE
5 STRUCTUREFUNCTION STUDIES ONCUTINASE A SMALL LIPOLYTIC ENZYME WITHA WATER ACCESSIBLE ACTIVE SITE
GENETICS STRUCTURE AND APPLICATIONS
7 SOLVENT ENGINEERING MODULATESSTEREOSELECTIVITY OF MICROBIAL LIPASES
8 STABILIZATION OF INDUSTRIAL ENZYMES BYPROTEIN ENGINEERING
13 ENGINEERING PROTEINS FOR THEDEGRADATION OF RECALCITRANT COMPOUNDS
14 PROTEIN ENGINEERING FOR BIOSENSORS
15 PROTEIN ENGINEERING IN VACCINEDEVELOPMENT
16 EXPLORING MOLECULAR RECOGNITION BYCOMBINATORIAL AND RATIONAL APPROACHES
17 PRODUCTION AND POTENTIALAPPLICATIONS OF RECOMBINANT GASTRICLIPASES IN BIOTECHNOLOGY
PROTEIN ENGINEERING OF A RASSPECIFICGUANINE
19 EXPRESSION AND USE OF TAGGED CELLSURFACE RECEPTORS FOR DRUG DISCOVERY
20 ESTROGEN RECEPTOR AS A TARGET FORNEW DRUG DISCOVERY
9 DESIGN OF BIOTECHNOLOGICALLYAPPLICABLE YEAST CARBOXYPEPTIDASE YMUTANTS WITH INCREASED PREFERENCE FORC...
10 ENGINEERINGbGLYCOSIDE HYDROLASES
11 ENGINEERING SPECIFICITY AND STABILITY INGLUCOAMYLASE FROMASPERGILLUS NIGER
12 PECTIN MODIFYING ENZYMES ANDDESIGNER PECTINS
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active site activity affinity amino acid analysis antibody antigen Aspergillus assay bacterial Biochem Biochemistry Biol Biotechnol Biotechnology Candida carboxylate carboxypeptidase catalysis catalytic catalytic triad CBDs cDNA cell lines cellulose characterization Chem chemical cloning coli complex compounds conformational change crystal structure cutinase Derewenda disulfide electrode enzymatic enzymes Escherichia estrogen expression fatty acid Figure function fusion protein gene genetic glucoamylase halide haloalkane haloalkane dehalogenase hydrogen bonds hydrolases hydrolysis hydrophobic identified immobilized inclusion bodies increased inhibitor interactions isoamylase kinetic ligand lipase lipid loop membrane molecular molecules mutagenesis mutants nucleophile oxyanion oxyanion hole P1 residues pectate lyase pectin peptide plasmid production protease protein engineering protein folding purification R-ECD reaction receptor recombinant protein region Rhizopus screening selected sequence serine side chain site-directed Skerra soluble solvent specificity stability Strep-tag streptavidin studies substrate binding surface Svensson synthesis target thermostable triglycerides vaccine vitro wild type wild-type