Protein Engineering and Design
Paul R. Carey
Academic Press, Jun 21, 1996 - Science - 361 pages
The design and production of novel peptides and proteins occupy pivotal positions in science and technology and will continue to do so in the 21st century. Protein Engineering and Design outlines the rapid advances in computer-based modeling, protein engineering, and methods needed for protein and peptide preparation and characterization. This indispensable reference lays the groundwork for understanding this multidisciplinary activity while providing an introduction for researchers and students to the field of protein design.
* Introduces and defines the techniques involved in protein engineering and design
* Provides a concise overview of key technologies involved and demonstrates their contributions to the specialized design and production of novel proteins and peptides
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Acad AChR AChR subunits activity affinity agonist amino acid amino acid sequence assay atoms backbone binding sites Biochem Biochemistry Biol Biophys cell Chem chemical cholinergic circular dichroism Clore codons coli complex conformational Conti-Tronconi copy number crystal cytoplasmic DeGrado diffraction disulfide bond domain enzyme expression extracellular folding four-helix bundle function GABAA GABAA receptor gene genetic glutamate glycine glycine receptor Gronenborn groups helices heterologous proteins hydrophobic interactions ion channel Kohn labeling levels ligand loop mammalian membrane Methods Enzymol molecular molecule muscle mutagenesis mutations Natl natural proteins Nature London neuronal AChRs o-BGT binding o-helices oligonucleotides plasmid poly(amino acids polymerase polymers polypeptide Proc production promoter protease protein structure protons Raman receptor recombinant protein residues resolution secondary structure secretion segment side chains signal peptide soluble specific spectra spectroscopy spectrum stability strategy studies subunits synthetic tion Torpedo AChR vectors yeast
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