Proteins LABFAX ; Edited by N. C. Price
Nicholas C. Price
BIOS Scientific Publishers, 1996 - Science - 318 pages
Proteins Labfax is a detailed and comprehensive compendium of information on protein chemistry, and the purification and physical characterization of proteins. The book provides data on all the procedures usedon a regular basis by researchers studying proteins, including chromatography, crystallization, size determination, computer-aided structure analysis, amino acid analysis, and the chemical modification of amino acid side chains. General data is also givenon buffers, salting out, criteria of purity, expression systems, and protein stability.The contents of Protein Labfax have been carefully chosen to complement the data presented in its companion volume, Enzymology Labfax.
* Serves as a companion volume to Enzymology Labfax
* Comprehensive coverage of protein chemistry and the purification and physical characterization of proteins
* Extensive information presented on procedures used regularly by researchers
* A complete one-stop source for biochemists and researchers in the field
What people are saying - Write a review
We haven't found any reviews in the usual places.
PREPARATION OF CELL EXTRACTS
Assays of proteins in unfractionated cell extracts
39 other sections not shown
absorption adsorbent affinity chromatography amino acid Anal analysis Analytical Ultracentrifugation antibodies antigen application assay beads binding Biochem Biochemistry Biol biological activity Biophys Biotechnol bonds Brocklehurst buffer cell centrifugation Chapter Chem chemical coefficient column components concentration containing coupling covalent cross-linking crystallization cysteine denaturation detection detergents dilute disulfide electrophoresis elution enzyme example extraction fusion partner fusion protein gel filtration gradient groups histidine HPLC hydrophobic inclusion bodies inhibitors interaction ion-exchange ionic strength isoelectric isoelectric point J-coupling ligand lysine matrix membrane proteins metal ions Methods Enzymol mg ml-1 mixture modification molecular mass molecules N-terminal Nucleic Acids peptide polypeptide precipitation procedure protein purification proteinases purity range reaction reagents recombinant protein refolding residues resonance salt sample SDS-PAGE secondary structure sedimentation sensitive separation sequence side-chains soluble solution solvent specific spectra spectroscopy substrate sulfate synthesis Table target protein technique temperature thiol volume