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35-mm Petri dish acid adult human antibody approx assay astrocytes beaker blocking buffer brain Bunge Cell Culture cell number cell suspension cerebellum cerebral hemispheres collagen concentration counting coverslips culture dishes culture medium curved forceps Dilute disaggregated distilled water DPBS embryo enzyme ethanol explants Fedoroff feeding medium filter filtration flask forceps ganglia gently glass Petri dish glia glial cells growth Hanks hemocytometer Incubate instruments isolated L-15 medium layer maintenance medium mesh method metrizamide mg/mL microexplant microglia motoneurons mouse myelination neopallium Neural Cell Culture neurites neurons Neurosci neutral red newborn Nitex Note oligodendrocytes Pasteur pipet Petri dish phosphate buffered saline Place plastic Petri dish plating efficiency poly-L-ornithine Preparation procedure protein Protocols for Neural Puck's BSS Remove Resuspend Rinse room temperature Schwann cells scissors spinal cord staining sterile stock solution Store supernatant surface syringe TAAB tissue culture trypsin Vellis viable cells vitro wash