Pulsed Field Gel Electrophoresis: A Practical Guide

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Academic Press, Dec 2, 2012 - Science - 272 pages
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Pulsed Field Gel Electrophoresis: A Practical Guide is the first laboratory manual to describe the theory and practice of this technique. Based on the authors' experience developing pulsed field gel instruments and teaching procedures, this book provides everything a researcher or student needs to know in order to understand and carry out pulsed field gel experiments. Clear, well-tested protocols assume only that users have a basic familiarity with molecular biology. Thorough coverage of useful data, theory, and applications ensures that this book is also a lasting resource for more advanced practitioners of pulsed field gels.

Key Features
* Reviews all types of pulsed field gel electrophoresis
* Describes all commercially available systems and summarizes advantages and limitations of each
* Includes step-by-step protocols for sample preparation and analysis
* Illustrated with photographs that depict
* How to run gels
* What the results should look like
* What they look like when they go wrong
* Covers applications to a wide range of organisms
* Includes bibliography of over 900 publications and cross-referenced by topic, application, and organism
 

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Contents

CHAPTER 1 INTRODUCTION TO CONVENTIONAL AND PULSED FIELD GEL ELECTROPHORESIS
1
CHAPTER 2 SETTING UP A PULSED FIELD GEL ELECTROPHORESIS SYSTEM
7
CHAPTER 3 PREPARATION OF DNA FOR PULSED FIELD ANALYSIS
25
CHAPTER 4 POURING GELS AND HANDLIN G SAMPLES
75
CHAPTER 5 RESTRICTION ENZYME DIGESTION OF LARGE DNA FRAGMENT SINAGAROSE
87
CHAPTER 6 SWITCH INTERVALS A N D RESOLUTION IN PULSED FIELD GELS
107
CHAPTER 7 FIELD INVERSION GEL ELECTROPHORESIS
121
CHAPTER 8 VARIABLES THAT AFFECT PULSED FIELD GELS
129
CHAPTER 10 SPECIAL APPLICATIONS
149
CHAPTER 11 TROUBLESHOOTING
167
SCHEMATIC DIAGRAM OF THE CHEF SYSTEM
177
SCHEMATIC DIAGRAM OF THE FIGE SYSTEM
189
SCHEMATIC DIAGRAM OF THE ELECTRICAL CIRCUIT FOR AVFIGE
191
BIBLIOGRAPHY OF PULSED FIELD GEL ELECTROPHORESIS
193
INDEX
245
Copyright

CHAPTER 9 SOUTHERN BLOT ANALYSIS OF PULSED FIELD GELS
141

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