Pulsed Field Gel Electrophoresis: A Practical Guide
Pulsed Field Gel Electrophoresis: A Practical Guide is the first laboratory manual to describe the theory and practice of this technique. Based on the authors' experience developing pulsed field gel instruments and teaching procedures, this book provides everything a researcher or student needs to know in order to understand and carry out pulsed field gel experiments. Clear, well-tested protocols assume only that users have a basic familiarity with molecular biology. Thorough coverage of useful data, theory, and applications ensures that this book is also a lasting resource for more advanced practitioners of pulsed field gels.
What people are saying - Write a review
We haven't found any reviews in the usual places.
CHAPTER 3 PREPARATION OF DNA FOR PULSED FIELD ANALYSIS
CHAPTER 4 POURING GELS AND HANDLIN G SAMPLES
CHAPTER 5 RESTRICTION ENZYME DIGESTION OF LARGE DNA FRAGMENT SINAGAROSE
CHAPTER 6 SWITCH INTERVALS A N D RESOLUTION IN PULSED FIELD GELS
CHAPTER 7 FIELD INVERSION GEL ELECTROPHORESIS
CHAPTER 8 VARIABLES THAT AFFECT PULSED FIELD GELS
CHAPTER 9 SOUTHERN BLOT ANALYSIS OF PULSED FIELD GELS
Other editions - View all
Acad agarose gels analysis bacterial Biol Birren buffer Cantor cells cells/ml cerevisiae CHEF circular DNA clones conidia culture different switch intervals DNA prepared DNA—agarose EDTA electric ﬁelds electrodes ethidium bromide field gel ﬁeld gel electrophoresis ﬁeld inversion gel FIGE ﬁrst gel box gel run gel strip gene Genet genome hybridization Incubate inversion gel electrophoresis karyotype lambda ladders lane large DNA molecules ligation linear long-range restriction map low melting agarose mammalian DNA markers mg/ml mobility molecular Natl nuclease Nucleic Acids Res partial digestion pellet PFGE phage physical map pipet plasmid plugs pombe procedure proteinase proteinase K protoplasts pulsed field pulsed ﬁeld gel ramping range region reorientation angle resolution restriction digestion restriction enzyme restriction map Resuspend room temperature separation solution speciﬁc spheroplasting strain switch interval TAE buffer transfer tube Turmel V/cm voltage gradient volume yeast chromosomes ZIFE Zymolyase