Quantitative Fluorescence Microscopy

Front Cover
Cambridge University Press, Jul 26, 1991 - Medical - 236 pages
Quantitative fluorescence microscopy is concerned with making measurements from fluorescent specimens in a fluorescence microscope, by measuring fluorescence emission from a defined area or areas of a specimen. This technique is most commonly used to determine the amount of some specific substance, such as DNA, in some particular area of a cell. But it has many other uses; for example, it can be used to identify certain substances in the cell by examining their fluorescence characteristics. This book is a complete guide to this technique for all biologists. It describes the principles and applications of quantitative fluorescence microscopy and also gives much practical information about the instrumentation required. There is also a discussion of the exciting developments in confocal fluorescence microscopy which allows the three dimensional distribution of particular substances to be determined. Everyone presently using this technique, or wishing to start using it will need to read this book.
 

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Contents

Principles of quantitative fluorescence microscopy
1
Instrumentation for microfluorometry
13
Microfluorometric technique
30
errors standardization and data processing
41
Applications of microfluorometry
59
Principles of microspectrofluorometry
67
microspectrofluorometers
73
Microspectrofluorometric technique
86
errors standardization and data processing
96
Applications of microspectrofluorometry
110
Timeresolved fluorescence phosphorescence and polarization
128
Quantitative enzyme studies
136
Flow cytometry by Hans J Tanke
162
History of quantitative fluorescence microscopy
179
Conversion table photon energy eV to wavelength nm
194
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Page 229 - Calcium gradients in single smooth muscle cells revealed by the digital imaging microscope using Fura-2.
Page 205 - Theoretical and experimental aspects of enzyme determination in a cytochemical model system of polyacrylamide films containing alkaline phosphatase. J Histochem Cytochem 15:631...
Page 207 - Irradiation of specimens by excitation light before and after staining with pararosaniline Feulgen: a new method to reduce non-specific fluorescence in cytofluorometry.
Page 205 - International Union of Pure and Applied Chemistry, Organic Chemistry Division, Commission on the Nomenclature of Organic Chemistry.
Page 207 - MANDELL. 1978. Fading: A new cytofluorimetric measure quantifying serotonin in the presence of catecholamines at the cellular level in brain.
Page 201 - Properties and kinetics of reduced pyridine nucleotide fluorescence of the isolated and in vivo rat heart.
Page 217 - Ostling, O. and Johanson, KJ (1984) Microelectrophoretic study of radiationinduced DNA damages in individual mammalian cells. Biochem. Biophys. Res. Commun. 123, 291-298.

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