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Kinetics of incorporation of HGTP
F Uptake of HGTP into acidsoluble pools
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acid-insoluble RNA Actinomycin-D adult mouse brain amanitin Biol buffer buffer solution cellulose cellulose column chromatin concentration cytoplasm day and adult effect enzyme eucaryotic Figure gene glial cell nuclei H-GTP into RNA histones Hn-RNA homopolymer incorporation of H-GTP incubated inhibited ionic conditions isolated nuclei labelled m-RNA M-sucrose Materials and Methods maturation-dependent messenger RNA mouse brain nuclei mouse brain tissue neonatal neuronal neuronal and glial non-histone proteins nuclear preparations nuclear RNA synthesis nuclei and nucleoli nuclei isolated nucleic acid nucleolar polymerase nucleoli nucleoplasmic nucleoplasmic polymerase nucleotide observed old mouse brain poly poly-A postnatal postnatal development precursor molecule presence of Mg radioactive precursor radioactivity rate of RNA reaction mixture ribonuclease ribosomal RNA metabolism RNA molecules RNA polymerase activity RNA products RNA species RNA synthesis RNA synthetic activity RNase sequences specific spermidine stages of postnatal sucrose t-RNA T.C. Johnson template transcription ug/ml whole brain