Receptor Binding Techniques
Anthony P. Davenport
Springer Science & Business Media, 2005 - Medical - 256 pages
A comprehensive collection of readily reproducible methods for studying receptors in silico, in vitro, and in vivo. These cutting-edge techniques cover mining from curated databases, identifying novel receptors by high throughput screening, molecular methods to identify mRNA encoding receptors, radioligand binding assays and their analysis, quantitative autoradiography, and imaging receptors by positron emission tomography (PET). This book equips the researcher with techniques for exploring the unprecedented number of new receptor systems now emerging and the so-called "orphan" receptors whose activating ligand has not been identified.
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5-HT3 receptor acid activity affinity agonist aliquot Amersham Biosciences amount analysis antisense approx assay buffer autoclaved autoradiography binding sites Bmax brain cDNA cells concentration coverslip cryostat curve database Davenport density DEPC-treated dH2O detected diluted edited emission emulsion endothelin enzyme ETB receptors ethanol expression film fluorescent fluorophores FRET fusion proteins G protein-coupled receptors G proteins gene genomic GPCRs GPR4 human hybridization incubation interactions labeled laser ligand method microscope slides molecular mRNA nonspecific Note Nuclear Receptor peptide Pharmacol phosphor imaging pipet plasma membrane primary antisera primers probe protein-coupled receptors protocol quantitative radioactivity radiolabeled radioligand radioligand binding reaction reagent riboprobe RNA samples room temperature scanning screen sequence signal solution SPA assays SPA bead specific staining stored Subheading TaqMan target Techniques tion tissue sections Tris-HCl trypan blue tube unlabeled wash