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ReceptorGprotein complexes in solution
Reconstitution of hormonesensitive adenylate
Reconstitution of related receptorcoupled signal
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3H]inositol a-subunits absorbance acid adenylate cyclase affinity agonist antibodies assay Biochem Biol buffer Ca2+ Calibration capacitance cells centrifugation Chem cholate chromatography Ci/mmol column concentration cyclic GMP described detergent digitonin dilution dissociation EDTA electrode elution Equation erythrocyte Extracti-gel extraction Figure filter filtration fluorescence fractions G-proteins giga-seal GTP-binding protein GTPase GTPase activity guanine guanine nucleotides headstage high-affinity incorporated incubation indicator inositol phosphates insulin receptor interactions intracellular isoproterenol labelled ligand lipid lipid vesicles Lubrol luminescence measured membrane method mg/ml MgCl2 microelectrodes mixture molecules muscarinic NaCl non-specific binding patch pellet pertussis toxin phosphatidylcholine phospholipid phospholipid vesicles phosphorylation phosphotyrosine photolysis photoproteins pipette pmol Practical Approach preparation presence probes procedure Protocol purified R-G complexes Receptor Biochemistry Receptor-Ligand reconstitution reference rhodopsin sample sensitivity signal sodium solubilized solution subunits supernatant techniques tracer ligand transducin Tris-HCl tubes tyrosine kinase vesicles vitro voltage volume wash wavelengths whole-cell