Recombinant DNA: Genes and Genomes: A Short CourseRecombinant DNA, Third Edition, is an essential text for undergraduate, graduate, and professional courses in Genomics, Cell and Molecular Biology, Recombinant DNA, Genetic Engineering, Human Genetics, Biotechnology, and Bioinformatics. The Third Edition of this landmark text offers an authoritative, accessible, and engaging introduction to modern, genome-centered biology from its foremost practitioners. The new edition explores core concepts in molecular biology in a contemporary inquiry-based context, building its coverage around the most relevant and exciting examples of current research and landmark experiments that redefined our understanding of DNA. As a result, students learn how working scientists make real high-impact discoveries. The first chapters provide an introduction to the fundamental concepts of genetics and genomics, an inside look at the Human Genome Project, bioinformatic and experimental techniques for large-scale genomic studies, and a survey of epigenetics and RNA interference. The final chapters cover the quest to identify disease-causing genes, the genetic basis of cancer, and DNA fingerprinting and forensics. In these chapters the authors provide examples of practical applications in human medicine, and discuss the future of human genetics and genomics projects. |
Contents
SECTION | 1 |
3 polyA Tails and 5 Methylguanosine | 3 |
Genes Are Mapped on Chromosomes Using Linkage | 7 |
Quantitating DNA Bases in Different Organisms Hints at Unlimited | 14 |
Information Flow from DNA to Protein | 29 |
Control of Gene Expression | 53 |
Eukaryotic Transcription and Gene | 62 |
Basic Tools of Recombinant DNA | 75 |
FOUNDATIONS OF GENOMICS | 247 |
Doublestranded RNA Is the Trigger | 253 |
Longer Sequences Are Put Together | 264 |
How the Human Genome Was Sequenced | 273 |
Comparative Sequence Analysis Shows That | 282 |
ANALYZING GENOMES | 309 |
Comparative Genomics Helps to Identify | 329 |
From Genome Sequence to Gene Function | 335 |
Fundamental Features of Eukaryotic Genes | 107 |
Caps Are Added to the Ends | 121 |
Southern and Northern Blotting Procedures | 126 |
A New Toolbox for Recombinant DNA | 129 |
Mobile DNA Sequences in the Genome | 159 |
Epigenetic Modifications of the Genome | 189 |
The Gene Xist Its Complement Tsix | 196 |
Not All Genes on the Mammalian X | 197 |
Imprinting Must Be Correct for the Proper | 210 |
RNA Interference Regulates Gene Action | 219 |
mRNA Levels Are Also Measured | 343 |
Arrayed Antibodies Are Used to Measure | 356 |
HUMAN GENOMICS | 365 |
Pharmacogenomic Strategy Leads | 387 |
Understanding the Genetic Basis of Cancer | 395 |
DNA Fingerprinting and Forensics | 431 |
Familial Searches Identify Suspects | 449 |
Figure Credits | 459 |
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Common terms and phrases
Acad activity allele amino acids analysis antibody bacteria base pairs binding Biol biological cancer cDNA cells chain Chapter chemical chromosome cloned codon Cold Spring Harbor complementary complex containing deletion DNA fragments DNA molecules DNA sequence DNA strands domain Drosophila EcoRI elements embryos encoded eukaryotic example exons FIGURE fluorescent function gene expression genetic genome sequence genomic DNA helix histone homologous human genome hybrid identified infection insertion introns isolated locus markers ment methylation mice microarray molecular mouse mRNA mutations Natl normal nucleic acid nucleotides oligonucleotide oncogene operon organisms phage plants plasmid poly(A polypeptide primers probe Proc produced promoter protein reaction recombinant DNA region regulation replication repressor restriction enzyme ribosome RNA polymerase RNAi samples segments siRNAs specific splicing ẞ-galactosidase ẞ-globin structure subunit synthesis target template tion trans transcription factors transgene transposons tRNA tumor vector viral virus viruses wild-type X chromosome yeast