Recombinant DNA Laboratory Manual, Revised Edition

Front Cover
Academic Press, Dec 2, 2012 - Science - 224 pages
0 Reviews
The latest edition of this introductory benchtop manual is up-to-date, affordable, and easy-to-follow. This text is perfect for your two-quarter or one semester course in Recombinant DNA Techniques and is specifically designed to lead your student or technician, who is a newcomer to molecular biology, from the basic skills of growing and maintaining bacterial colonies through plasmid DNA isolation, cloning, DNA sequencing, and hybrid detection.
  • Comb-bound, three-column, large 9-1/4" x 7-1/2" format
  • Exercises contain explanatory material and margin notes that pinpoint critical steps and important concepts
  • Necessary reagents and equipment are presented in a checklist at the beginning of each protocol
  • Techniques for bacteria are complemented with those for Drosophila
  • Each experiment has been tested in the laboratory by students for five years
  • Features a complete chapter on computers in the molecular biology laboratory
  • Presents helpful appendixes on safety in the laboratory, frequently used ancillary techniques, and recipes for buffers, media, and strains
 

What people are saying - Write a review

We haven't found any reviews in the usual places.

Contents

Lab I BACTERIAL GROWTH PARAMETERS
1
Lab II Isolation and Analysis of Bacterial and Drosophila Chromosomal DNA
11
Lab III Plasmid DNA Isolation and Agarose Gel Analysis
31
Lab IV Introduction of DNA into Cells
43
Lab V Tn5 Mutagenesis of PBR329
51
Lab VI DNA Cloning in M13
67
Lab VII DNA Sequencing
97
Lab VIII DNA Gel Blotting Probe Preparation Hybridization and Hybrid Detection
117
USE OF COMPUTERS IN A MOLECULAR BIOLOGY LABORATORY
171
SAFETY IN THE RECOMBINANT DNA LABORATORY
181
SOLUTIONS
187
STRAINS AND DNA
191
MOLECULAR BIOLOGY REAGENT SUPPLIERS
193
FREQUENTLY USED ENZYMES
195
FREQUENTLY USED TECHNIQUES NOT DESCRIBED IN LABS
199
INDEX
219

Lab IX Lambda Phage Manipulations
135
Lab X The Polymerase Chain Reaction Technique
151

Other editions - View all

Common terms and phrases

Bibliographic information