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Preparation of Neuronal Perikarya by Micromanipulation
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0.32 M sucrose Acta activity adult rats amino acids analysis assay astrocytes Biochem Biol Biophys brain tissue buffer carbohydrate cell nuclei cerebral cortex Chem chondroitin chromatography Cohen concentration containing Cotman cytoplasmic density differences density gradient dermatan sulfate described detergent determination dry weight eluted enzyme extracellular space extract fluid fluorescence fraction g/ml gangliosides glycopeptides glycoproteins Harreveld hexosamines histamine homogenate hyaluronidase hydrolysis incubation inulin isopycnic isopycnic banding labeled Lajtha layer lipid measured medium membranes methanol method microsomal mitochondria mixture mucopolysaccharides myelin needle nervous tissue Neurochem neurons obtained oligosaccharides particle density pellet perfusion pH 5 enzyme Physiol polyribosomes prepared procedure purified rat brain reaction removed resolution rotor sample second marker space sedimentation coefficients separation sialic acid slices sodium solution solvent subcellular particles substrate sucrose gradient sugars supernatant suspension synaptosomes technique temperature tion trypsin tube values vitro vivo volume white matter Zomzely