Scale-Up and Automation in Plant Propagation: Cell Culture and somatic cell Genetics of Plants

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Indra Vasil
Academic Press, Dec 2, 2012 - Science - 292 pages
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Scale-Up and Automation in Plant Propagation reviews methods of automation and scale-up of plant propagation in vitro. It looks at the large scale clonal propagation of plants, or micropropagation, as the first major practical application of plant biotechnology. It also discusses the advantages and limitations of micropropagation and evaluates current methods of commercial micropropagation.
Organized into 13 chapters, this volume begins with an overview of the benefits of scaling up and automating plant propagation before proceeding with a discussion of synthetic seeds and their use for plant propagation, along with problems and economic considerations associated with synthetic seed technology. It then considers the implementation of somatic embryogenesis technology for clonal forestry, the development and commercialization of bioreactor technology for automated propagation of potato microtubers and lily microbulbs, and approaches to automated propagation of fruit trees. Other chapters focus on issues of cost reduction and development of ""new"" products, scale-up and operation of prototype bioreactors for plant propagation, and application of machine vision technology to scale-up and automated evaluation of somatic embryogenesis in sweet potato. The book also describes methods of measurement and control of the environment in culture, environmental factors affecting photosynthesis, and use of robotics and field transplanters in the automation of plant propagation.
Scientists and plant breeders will find this book extremely useful.
 

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Contents

Chapter 1 Rationale for the ScaleUp and Automation of Plant Propagation
1
Chapter 2 Current Methods of Commercial Micropropagation
7
Chapter 3 Synthetic Seed Technology
35
Chapter 4 Development of an Embryogenie System for Automated Propagation of Forest Trees
75
Chapter 5 Automated Propagation of Microtubers of Potato
95
Chapter 6 Automated Propagation of Microbulbs of Lilies
111
Chapter 7 Problems and Perspectives for the Automated Propagation of Fruit Trees
133
Chapter 8 Bioreactors and Image Analysis for ScaleUp and Plant Propagation
147
Chapter 9 Automated Evaluation of Somatic Embryogenesis in Sweet Potato by Machine Vision
179
Chapter 10 Control of Contamination in Automated Plant Protopagation
197
Chapter 11 Controlled Environments in Conventional and Automated Micropropagation
213
Chapter 12 The Use of Robotics in Automated Plant Propagation
231
Chapter 13 The Use of Automated Field Transplanters in Plant Propagation
245
Index
261
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