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SELECTION AND PHENOTYPIC CHARACTERIZATION OF CHLORATERESISTANT
Materials and Methods
4 other sections not shown
20mM chlorate ammonium anthers Aspergillus nidulans assay auxotrophs callus cell cultures Cells on Nitrate Chaleff chlorate Chlorate-resistant Cell Lines chlorate-resistant variants chlorate-sensitive chromosomes Clr cells clr30 cells clrl5 and clr30 clrl9 cells clrO and clrl9 clrO plants colonies complementation concentration cultured cells Datura innoxia diploid experiment extracts Figure flowers genetic haploid higher plants homozygotes homozygous hydroxyurea incubated induced isolated large number markers Mendel mesophyll mesophyll tissue metaphases Molec Muller mutagen mutants NADH-NR activity NH4-2 medium Nicotiana tabacum nitrate assimilation nitrate reductase activity nitrate uptake Nitrate-nonutilizing nitrate-utilizing nitrogen source NO3 medium normal plants parasexual Parental Cells Petri plates phenotype plant cells plants regenerated plastic Petri plates PmRl Gut protoplasts pollen polyploidy progeny protoplast fusion protoplasts regenerated plants resistance to chlorate somatic hybrid plants somatic hybrids source of nitrogen succinic acid suspension cultures Suspension-cultured cells utilization variant cell lines variant phenotype vitro whole plants xanthine oxidase