Stem Cell Culture

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Jennie P. Mather
Academic Press, Sep 2, 2011 - Science - 416 pages
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The purpose of Stem Cell Culture is to provide a comprehensive resource for researchers in the fields of embyonic, fetal and adult stem cell biology to find methods for the purification, culture, and differentitation of these cell types, with the main emphasis on the maintenance of the stem cell phenotype in vitro. This volume will be the first to broadly cover multiple types of stem cell culture from different ages, organs and species. Authors will focus on the practical do's and don'ts of isolating and culturing these cell types, and feel free to use illustrative data or diagrams whereever this improves the comprehension of the reader. This should allow the reader to compare and contrast techniques and make this a standard reference for those in the field, or desiring to start stem cell culture.
  • Describes techniques in stem cell research
  • Delineates critical steps and potential pitfalls for each method
  • Covers specific procedures in dealing with Human Embryonic Stem Cells

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Derivation of Human Embryonic Stem Cells in Standard and Chemically Defined Conditions
Autogeneic Feeders for the Culture of Undifferentiated Human Embryonic Stem Cells in Feeder and FeederFree Conditions
Microporous Membrane Growth Substrates for Embryonic Stem Cell Culture and Differentiation
Culture of Rodent Spermatogonial Stem Cells Male Germline Stem Cells of the Postnatal Animal
Characterization of Human Amniotic Fluid Stem Cells and Their Pluripotential Capability
Method to Isolate MesenchymalLike Cells from Whartons Jelly of Umbilical Cord
Isolation Characterization and Differentiation of Human Umbilical Cord Perivascular Cells HUCPVCs
Hepatic Stem Cells and Hepatoblasts Identification Isolation and Ex Vivo Maintenance
Isolation of Human Mesenchymal Stem Cells from Bone and Adipose Tissue
Culture of Mesenchymal StemProgenitor Cells in AdhesionIndependent Conditions
Purification and LongTerm Culture of Multipotent Progenitor Cells Affiliated with the Walls of Human Blood Vessels Myoendothelial Cells and Peric...
Isolation and Culture of Colon Cancer Stem Cells
Isolation and Establishment of Human Tumor Stem Cells
Stem Cells from Cartilaginous and Bony Fish
Volumes In Series

Culture of Pluripotent Neural Epithelial Progenitor Cells from E9 Rat Embryo
Primary and Multipassage Culture of Human Fetal Kidney Epithelial Progenitor Cells

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About the author (2011)

Mather-Genentech, Inc., San Francisco, CA

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