Stem Cell Culture

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Jennie P. Mather
Academic Press, Sep 2, 2011 - Science - 416 pages
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The purpose of this volume is to provide a comprehensive resource for researchers in the fields of embyonic, fetal and adult stem cell biology to find methods for the purification, culture, and differentitation of these cell types, with the main emphasis on the maintenance of the stem cell phenotype in vitro. This volume will be the first to broadly cover multiple types of stem cell culture from different ages, organs and species. Authors will focus on the practical do's and don'ts of isolating and culturing these cell types, and feel free to use illustrative data or diagrams whereever this improves the comprehension of the reader. This should allow the reader to compare and contrast techniques and make this a standard reference for those in the field, or desiring to start stem cell culture.

* Describes techniques in stem cell research
* Delineates critical steps and potential pitfalls for each method
*covers specific procedures in dealing with Human Embryonic Stem Cells
 

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Contents

Derivation of Human Embryonic Stem Cells in Standard and Chemically Defined Conditions
1
Autogeneic Feeders for the Culture of Undifferentiated Human Embryonic Stem Cells in Feeder and FeederFree Conditions
15
Microporous Membrane Growth Substrates for Embryonic Stem Cell Culture and Differentiation
29
Culture of Rodent Spermatogonial Stem Cells Male Germline Stem Cells of the Postnatal Animal
59
Characterization of Human Amniotic Fluid Stem Cells and Their Pluripotential Capability
85
Method to Isolate MesenchymalLike Cells from Whartons Jelly of Umbilical Cord
101
Isolation Characterization and Differentiation of Human Umbilical Cord Perivascular Cells HUCPVCs
121
Hepatic Stem Cells and Hepatoblasts Identification Isolation and Ex Vivo Maintenance
137
Isolation of Human Mesenchymal Stem Cells from Bone and Adipose Tissue
257
Culture of Mesenchymal StemProgenitor Cells in AdhesionIndependent Conditions
279
Purification and LongTerm Culture of Multipotent Progenitor Cells Affiliated with the Walls of Human Blood Vessels Myoendothelial Cells and Peric...
295
Isolation and Culture of Colon Cancer Stem Cells
311
Isolation and Establishment of Human Tumor Stem Cells
325
Stem Cells from Cartilaginous and Bony Fish
343
Index
369
Volumes In Series
379

Culture of Pluripotent Neural Epithelial Progenitor Cells from E9 Rat Embryo
227
Primary and Multipassage Culture of Human Fetal Kidney Epithelial Progenitor Cells
241

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About the author (2011)

Mather-Genentech, Inc., San Francisco, CA

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