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Immunoalkaline Phosphatase Technique
Application of AvidinBiotin Complex Technique
Microinjection and Immunofluorescence Microscopy
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absorbance acid amount antigen APAAP technique Bendayan binding sites buffer pH Cell Biol cell-surface citrate colloidal gold colloidal silver concentrations coverslip cryostat cryostat sections culture supernatant Cytochem detected dilution distilled water electron microscopic embedding enzyme enzyme-gold complex Epon ethanol fixation fixed fluorescence formaldehyde frozen sections glutaraldehyde gold markers gold particles granules Histochem Histochemistry Horisberger immunoblotting immunocytochemical immunocytochemistry immunofluorescence Immunol immunoperoxidase immunostained incubation injected labeling lectin lectin-gold complex light microscopic localization macrophages method microinjection microtubules molecules monoclonal antibodies monoclonal antibody binding needle nitrocellulose nitrocellulose paper nonspecific obtained optimal ovomucoid pancreatic parabenzoquinone paraffin sections paraformaldehyde peptides peritoneal macrophages peroxidase Petrusz phosphate buffer pituitary Polak polypeptides preparation primary antibody procedure protein A-gold reaction product reagents receptor Rinse RNase-gold room temperature Rosset Roth samples SDS-PAGE second antibody semithin slides smears sodium solution specific spectrin staining substrate surface thin sections tissue sections tubulin vinculin Vonlanthen washed