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Determination of Carboxymethylcysteine
l2 Proteolytic Digestions
2 other sections not shown
0.2 M phosphate 0.l M mercaptoethanol 0.l M Tris 4PS-ribonuclease absorbance Aliquots alkylate amino acid Anfinsen assay autoxidation Biorex 70 buffers Cation-Exchange Chromatography cCMP chromatographed circular dichroic column of Biorex conformational change conformational isomers cytidine decrease denaturation determined dialysis dialyzed diethanoldisulfide digests disulfide bonds disulfide bridges enhancement of ribonuclease enzymatic activity enzyme in Fraction enzyme species extinction coefficient Figure free sulfhydryls gel filtration histidine hydrolysis Hydrolysis of cCMP increase incubated with 0.l incubation incubation of ribonuclease iodoacetate lyophilized maximal activity mercaptoethanol mercaptoethanol concentration Michael is constant mixed disulfide native enzyme native ribonuclease open beakers oxidation partially reduced peptides phosphate pK values plotted protein concentration protein in Fraction reaction mixture reoxidation residues ribonuclease activity room temperature Rosario & Hammes sample species with enhanced specific activity spectra spectrophotometrically Steady-State Kinetics studies tertiary structure thiol concentration titration turnover number tyrosine uridine Watkins & Benz