The Metabolic Pathway Engineering Handbook: Tools and Applications

Front Cover
Christina Smolke
CRC Press, Jul 28, 2009 - Science - 581 pages
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This second volume of the Metabolic Pathway Engineering Handbook delves into evolutionary tools and gene expression tools for metabolic pathway engineering. It covers applications of emerging technologies including recent research genome-wide technologies, DNA and phenotypic microarrays, and proteomics tools for experimentally determining flux through pathways. This volume also looks at emerging applications for producing fine chemicals, drugs, and alternative fuels.

Christine Smolke, who recently developed a novel way to churn out large quantities of drugs from genetically modified brewer’s yeast, is regarded as one of the most brilliant new minds in biomedical engineering. In this handbook, she brings together pioneering scientists from dozens of disciplines to provide a complete record of accomplishment in metabolic pathway engineering. With a wealth of cutting edge research and analysis, this work also serves as an invaluable resource for those seeking to add their own contributions. Organized by topic, this 3000 page reference is available as two volumes that can be purchased individually or as a set.

 

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Contents

Chapter 1 Evolutionary Engineering of Industrially Important Microbial Phenotypes
1-1
Chapter 2 Improving Protein Functions by Directed Evolution
2-1
Chapter 3 Engineering DNA and RNA Regulatory Regions through Random Mutagenesis and Screening
3-1
Chapter 4 Evolving Pathways and Genomes for the Production of Natural and Novel Compounds
4-1
Chapter 5 Models Predicting Optimized Strategies for Protein Evolution
5-1
Gene Expression Tools for Metabolic Pathway Engineering
5-29
Chapter 6 LowCopy Number Plasmids as Artificial Chromosomes
6-1
Chapter 7 Chromosomal Engineering Strategies
7-1
Future Prospects in Metaboli Engineering
14-13
Chapter 15 Systems Biology GenomeScale Models and Metabolic Engineering
15-1
Chapter 16 CellFree Systems for Metabolic Engineering
16-1
Chapter 17 In Silico Models for Metabolic Systems Engineering
17-1
Tools for Experimentally Determining Flux through Pathways
17-25
Chapter 18 GCMS for Metabolic Flux Analysis
18-1
Chapter 19 Tools for Measuring Intermediate and Product Formation
19-1
Chapter 20 Use of AEXHPLCESIMS for 13CLabeling Based Metabolic Flux Analysis in Saccharomyces cerevisiae and Penicillium chrysogenum
20-1

Chapter 8 Regulating Gene Expression through Engineered RNA Technologies
8-1
Chapter 9 Tools Designed to Regulate Translational Efficiency
9-1
Chapter 10 Metabolic Engineering of the Secretory Processing Pathway in Eukaryotes
10-1
Chapter 11 Engineering Multifunctional Enzyme Systems for Optimized Metabolite Transfer between Sequential Conversion Steps
11-1
Chapter 12 Practical Pathway EngineeringDemonstration in Integrated Tools
12-1
Application of Emerging Technologies to Metabolic Engineering
12-15
DNA Microarrays Phenotypic Microarrays and Proteomics
13-1
Chapter 14 Monitoring and Measuring the Metabolome
14-1
Future Applicationf of Meabolic Engineering
VE-1
Chapter 21 Energy and Cofactor Issues in Fermentation and Oxyfunctionalization Processes
21-1
An Emerging Technology
22-1
Chapter 23 Applications of Metabolic Engineering for Natural Drug Discovery
23-1
Chapter 24 Metabolic Engineering for Alternative Fuels
24-1
Back cover
24-29
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