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Chemical shift assignments for juglone
The natural abundance cmr spectrum of mollisin
7 other sections not shown
acetate concentration acetic anhydride addition anthraquinone biosynthetic C]-enriched caesia carbons chemical shift Chemical shift assignments chloroform extracts chromophore cmr spectrum concentrated in vacuo containing coupling constants crude extract culture plates culture tubes cultures were grown daunomycinone dextrose distilled H2O dried emodin enriched experiments extracted with chloroform extracted with Skelly Extraction procedure extracts were pooled flasks were added fractions gm of islandicin gm of lipid gm of mollisin growth conditions growth period indicated a purity Inoculation and pulsing islandicin triacetate juglone lipid material malt extract agar metabolite methanol mg/ml mixture ml aliquot mmole molecule mollisin acetate mycelial natural abundance obtained peak petri dishes prepared previously described pulsing procedures pyridine Radioactivity assay recrystallized red solid red-orange oil sample silica gel sodium acetate solutions spectra spore suspension spores sublimed tions torr to give UV-VIS vacuo to yield visible spectrum yellow yellow-brown solid