The Immunoassay Handbook

Front Cover
David Wild
Gulf Professional Publishing, 2005 - Medical - 930 pages
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Immunoassays are biochemical tests that rely on antibodies (a special type of protein) to bind to specific molecular structures (antigens). They have become the most commercially successful technology in diagnostics, from home pregnancy testing kits to AIDS testing.
Highly illustrated and clearly written, The Immunoassay Handbook provides an excellent guide to this fast-growing industry.

Revised and updated, this third edition offers a balanced review of practical information and clinical applications in the field of immunoassay technology.

Comprehensive and expertly written, The Immunoassay Handbook will be invaluable for a wide range of users from researchers developing immunoassay systems to developmental and marketing staff who need an excellent overview of how immunoassay principles, systems and applications work.

With 68 chapters covering a wide range of topics, and new chapters looking at: surface plasmon resonance in kinetic, concentration and
binding site analyses, and the foundations of immunochemistry, this handbook is a must read for all laboratory researchers and academics who
use immunoassay as a tool including: pathologists, clinical and veterinary chemists, biochemists, students and researchers in immunology,
biochemistry and medicine.

  • This third edition contains updated and new information on advanced technology, including micro- and nanoscale immunoassays.
  • Provides a unique mix of practical information coupled with a review of clinical applications, and practical examples
  • Include contributions from world experts including Robert Ritchie and Professor Colin Self.
 

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Contents

Introduction to Immunoassay Principles Chris Davies
3
ADVIA Centaur Laura Taylor
33
NonCompetitive Immunoassays for Small Molecules The AntiComplex
41
Ambient Analyte Assay Roger Ekins
48
Free Analyte Immunoassay Nic D Christofides
63
The Foundations of Immunochemistry Robert F Ritchie
79
Concepts Chris Davies
103
Immunoassay Development in the In Vitro Diagnostic Industry Doug Brandt
136
CEDIA a Homogeneous Enzyme Immunoassay System William A Coty
412
VITROS ImmunoRate and MicroTipAssays
418
The TRIAGE System Kenneth F Buechler
424
Stratus CS Acute CareDiagnostic System W N McLellan
431
Persona Keith May
438
Laboratory Quality Assurance Pierre Blockx and Manuella Martin
456
PointofCare Testing James H Nichols
472
Immunoassay Troubleshooting Guide David Wild
483

Antibodies Eryl Liddell
144
Antibody fragments
154
Antibody conjugates and fusion proteins in diagnostics
161
Standardization and Calibration David Wild
167
Calibration
173
Signal Generation and Detection Systems Excluding Homogeneous Assays
192
Direct fluorescence
199
Homogeneous Immunoassays Edwin F Ullman
212
Calibration Curvefitting Barry Nix and David Wild
233
Conjugation Methods Alastair H Dent
246
Immunological Biosensors James K Gimzewski Jason Reed Michael A Teitell
265
Surface Plasmon Resonance in Kinetic Concentration and Binding Site Analyses
281
LabonaChip Micro and Nanoscale Immunoassay Systems Larry J Kricka
294
Introduction to Product Technology in Clinical Diagnostic Testing David Wild
313
Market Trends David Huckle and David Wild
319
Choosing an Automated Immunoassay System Lori J Sokoll and Daniel W Chan
333
Automated System Features Alain Truchaud Tanguy Le Neel Murielle Cazaubiel
338
MicroTrak Tom Houts
349
UniCAP 100 Gareth Evans
358
IMMULITE and IMMULITE 1000
370
AxSYM Theresa Donahoe
379
Elecsys Immunoassay Systems
385
Vitros ECi David Wild
391
IMMULITE 2000 and IMMULITE 2500
398
Elvio Gramignano
402
Thyroid Rhys John Carole A Spencer Nic Christofides and David Wild
523
The Adrenal Cortex Sami Medbak
544
Bone Metabolism Kay W Colston and John C Stevenson
552
Infertility Michael J Wheeler
565
In Vitro Fertilization and Embryo Transfer IVFET August C Olivar
580
Hirsutism and Virilization in the Female Michael J Wheeler
586
Pregnancy Tim Chard
598
Growth and Growth Hormone Deficiency Michael Preece and Jane Pringle
611
Diabetes Mellitus Penny Clark
617
Hematology Derek Dawson Harry Waters and John Ardern
629
Cardiac Markers Alan H B
649
Cancer Markers Mavanur R Suresh
664
Allergy Lars Yman
695
Quality control
706
Autoimmune Disease David F Keren
712
Sexually Transmitted Diseases Bruce J Dille Alan S Armstrong and Isa K Mushahwar
733
Congenital Diseases of Microbiological Origin Bruce J Dille John W Safford
746
Hepatitis Isa K Mushahwar
756
Human Retroviruses George J Dawson and Isa K Mushahwar
771
Dengue Virus Infections Bruce J Dille and Isa K Mushahwar
779
Therapeutic Drug Monitoring TDM Philip A Routledge and Alun D Hutchings
798
Drugs of Abuse Brian Widdop
816
Assays for Drugscreening Applications and Research Jeffrey K Horton
854
Immunoassay Applications in Veterinary Diagnostics Erwin Workman
885
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About the author (2005)

David Wild is a healthcare industry veteran, with experience in biotechnology, pharmaceuticals, medical devices and immunodiagnostics, which remains his passion. He worked for Amersham, Eastman-Kodak, Johnson & Johnson, and Bristol-Myers Squibb, and consulted for diagnostics and biotechnology companies. He led research and development programs, design and construction of chemical and biotechnology plants, and integration of acquired companies. Director-level positions included Research and Development, Design Engineering, Operations and Strategy, for billion dollar businesses. He retired from full-time work in 2012 to focus on his role as Editor of The Immunoassay Handbook, and advises on product development, manufacturing and marketing.

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