Time-of-flight Mass Spectrometry: Instrumentation and Applications in Biological Research

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American Chemical Society, Jan 1, 1997 - Science - 326 pages
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This book presents the basic principles of time-of-flight (TOF) mass s pectrometry with a strong emphasis on applications in biological resea rch. It describes many innovative techniques, including orthogonal ext raction, post source decay, and delayed extraction, and surveys ioniza tion techniques used on TOF mass spectrometers such as electron impact, plasma desorption, SIMS, FAB, laser desorption, MALDI, and electrosp ray. The book features chapters on applications to peptides/proteins, oligonucleotides, and other biological macromolecules, and examines ho w techniques might be used in sequencing the human genome.

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