Tissue In Situ Hybridization: Methods in Animal Development

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Wiley, Feb 5, 1997 - Science - 132 pages

Methods in Animal Development

Trevor Jowett

The European Molecular Biology Organization (EMBO) course on tissue in situ hybridization in animal developmental biology has served as an important and highly respected forum for the latest advances in the methodology of this valuable research tool. Developed from EMBO course materials, Tissue

In Situ Hybridization provides scientists and researchers worldwide with detailed coverage of new approaches, techniques, and protocols, along with up-to-date information on more established procedures.

Focusing particularly on the two-color in situ hybridization method to whole-mount embryos and tissue sections, this practical resource also compares different methods of producing differentially colored signals, and includes the results of protocol experiments with fluorescent and other alternative in situ hybridization techniques. Special features include:

  • Photographic examples—including color plates—to complement the text
  • Clear explanations of the principles underlying different methods
  • Detailed discussion and comparison of the different methods
  • Valuable troubleshooting advice and practical guidance
  • Comprehensive index, allowing quick and easy access to specific topics

Compiled by a leading expert in the field, Tissue In Situ Hybridization is an indispensable asset to professionals and researchers working in the areas of developmental, cell, and molecular biology.

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Probe Synthesis
Hybridization to WholeMount Embryos
Immunolocalization of Probes

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