Transgenic Animal Technology: A Laboratory Handbook
Carl A. Pinkert
Academic Press, Dec 2, 2012 - Science - 364 pages
Transgenic animal technologies and the ability to introduce functional genes into animals have revolutionized our ability to address complex biomedical and biological questions, as well as to make practical applications to production agriculture. This well-illustrated handbook covers the technical aspects of gene transfer in animals--from molecular methods to whole animal considerations--for the important laboratory and domestic animal species. It describes methodologies as employed by leading laboratories and is a key resource for researchers, as well as a tool for training technicians and students.
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Production of Transgenic Laboratory and Domestic Animal Species
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Acad analysis assay Biol blastocyst bone marrow Brinster cage Cell Cambridge cell line chicken chromosome colonies copy number culture detected difﬁcult dish donors efﬁciency eggs electroporation embryonic stem cells endogenous enzyme estrus exogenous females fertilization ﬁbroblasts ﬁrst gene expression gene therapy gene transfer genetic genome germ line growth hormone hematopoietic hepatocytes holding pipette homologous recombination human hybridization identiﬁcation inbred injection pipette insertion integration Laboratory liver luciferase males mating medium method microinjection modiﬁed mouse embryos mRNA murine mutation Natl Nature London needle offspring oviduct ovulation packaging cell Palmiter pigmentation Pinkert pipette plasmid probe Proc procedure production of transgenic pronucleus protein protocols rabbit recipient reporter gene retroviral vectors sequences serum signiﬁcant Southern blot speciﬁc sperm strains strategy superovulated techniques tion tissue titer trans transcription transfection transformed transgene expression transgenic animals transgenic families transgenic mice transplantation tube tumors tyrosinase viral virus vitro vivo