Troubleshooting Histology Stains
This book aims to solve the problems encountered by the laboratory worker when a particular histological staining process either goes wrong or generates a problematic result. The most common procedures are described in detail, and background information on the dyes and techniques is supplied. Variants are then discussed and, in the third section, the ease of use of the method, and the pitfalls are assessed and described. Well illustrated in color.
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Acid hematin for phospholipids
Adenosine triphosphatase ATPase demonstration
Chloroacetate esterase for mast and myeloid cells
48 other sections not shown
acid dye Alcian Blue alcohol Aldehyde Aldehyde Fuchsin alkaline anionic azodye Background information Background reading background staining Bancroft & Stevens basic dyeing batch cationic dye cells Chayen & Bitensky collagen commercial Common variants conjugated system contain counterstain cryosections dehydration diazonium salt differentiation dye-bath easy to carry enzyme ethanol extraction Fast Blue fixation fixative formalin glycolmethacrylate hematin hematoxylin Hexazotized Pararosanilin Horobin hydrophilic hydrophilic resin hydrophobic ions keeping reliable reagents large conjugated system lipids method Methyl Green Methylene Blue mountants Nile Blue Nile Red Obtaining and keeping occur Orcein oxidation paraffin paraffin sections Pararosanilin Plate positive control poststaining precipitate prepared prior to staining procedure easy processing are suitable proteins Pyronin reaction product routine control procedures Schiff reagent solu solvent specimen processing Stain or staining staining solution stains unexpectedly weakly substrate temperature tion Tissue stains unexpectedly Try extending types of specimen unexpected color Unexpected structures stain visualizing agent