Ubiquitin and Protein Degradation, Part 1

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Raymond J. Deshaies
Gulf Professional Publishing, 2005 - Science - 609 pages
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Since the inception of the series, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. The series contains much material still relevant today - truly an essential publication for researchers in all field of life sciences.

Ubiquitin and Protein Degradation, Part A will cover high level purification, bioinformatics analysis and substrate identification of the major proteins involved in protein degradation. The chapters are highly methodological and focus primarily on purification and analysis.

Topics include:
E1 Enzymes
E2 Enzymes
E3 Enzymes
Proteasomes
Isopeptidases
 

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Contents

of the El for Human NEDD8 the Brenda A Schulman
9
A Fluorescence Resonance Energy Transfer GuiLLAUME BOSSIS
20
Purification and Assay of the Chaperone Shigeo Murata
22
Properties of the ISG15 El Enzyme UbElL Robert M Krug
32
Atomic Force Microscopy of the Proteasome Pawel A Osmulski
34
Purification and Properties of the Ubiquitin Christopher Ptak
43
Functional Annotation of Deubiquitinating Annette M G Dirac
45
Expression Purification and Properties of the Kevin L Lorick
54
Purification and Analysis of Recombinant 1 IS Eugene I Masters
306
Purification and Assay of Proteasome Vicenca Ustrell
321
Purification Crystallization and XRay Michael Groll
329
Preparation of Hybrid 19S20SPA28 Paolo Cascio
336
Characterization of the Proteasome Using Suzanne Elsasser
353
Monitoring Activity and Inhibition of 26S Alexei F Kisselev
364
The Synthesis and Proteasomal Degradation of Y Amy Lam JenWei Huang
379
Assaying Degradation and Deubiquitination Rati Verma
391

Purification and Activity Assays for Ubc9 the Ali A Yunus
74
Expression and Evaluation of RING Yili Yang Kevin L Lorick
103
Expression and Assay of HECT Domain Sylvie Beaudenon
112
HighLevel Expression and Purification of Ti Li Nikola P Pavletich
125
In Vitro Reconstitution of SCF Substrate Matthew D Petroski
143
Expression and Assay of GlycoproteinSpecific Yukiko Yoshida
159
Affinity Purification of Mitotic Anaphase AVRAM HERSHKO
170
Purification and Assay of the Budding Yeast Lori A Passmore
195
Enzymology of the AnaphasePromoting Christopher W Carroll
219
Identification of Cell CycleDependent Franz Herzog
231
A TwoState XUELIAN LUO
246
Identification Expression and Assay of an Kazuhiro Iwai
256
Methods for the Functional Genomic Analysis Wei Li Sumit K Chanda
280
Purification of PA700 the 19S Regulatory George N DeMartino
295
Probing the UbiquitinProteasome System Martin A Hoyt
399
Characterization of Noncompetitive Maria Gaczynska
425
Development and Use of Antiproteasome Klavs B Hendil
439
Preparation and Characterization of Yeast and ShyrJiann
457
Purification of the COP9 Signalosome from Suchithra menon
468
Purification Method of the COP9 Signalosome Bettina K J Hetfeld
481
UBP43 an ISG15Specific Deconjugating Keun II Kim
491
Strategies for Assaying Deubiquitinating Sung Hwan Rang
500
In Vitro Cleavage of Nedd8 from Cullin 1 by Kosj Yamoah Kenneth
509
UbiquitinOvomucoid Fusion Proteins Tingting Yao
522
Using Deubiquitinating Enzymes as Rohan T Baker
540
Author Index
569
Subject Index
597
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