Wound Healing: Methods and Protocols

Front Cover
Luisa A. DiPietro, Aime L. Burns
Springer Science & Business Media, 2003 - Health & Fitness - 467 pages
1 Review
During the past decade the significant progress made in cell and molecular biotechnology has led to exciting discoveries in the field of tissue repair, producing a wealth of information about the healing process. In Wound Healing: Methods and Protocols, expert researchers and physicians not only describe classic and contemporary laboratory methods for studying tissue repair using a broad range of wound healing models, but also provide sensitive assays for the assessment of tissue healing. The systems detailed include human, animal, in vitro, in vivo, and impaired healing models, as well as models in which underlying systemic and genetic conditions influence the healing process. Useful for the study of many biological processes, including angiogenesis, epidermal differentiation and repair, acute inflammation, ECM synthesis, and remodeling, these powerful models illustrate biochemical, molecular, and surgical techniques designed for the analysis and manipulation of the healing wound, and provide step-by-step instructions to ensure successful results. In many cases, several different approaches to a single process are examined, with sample results and analyses given to help users in selecting the approach most suited to the problem at hand. Comprehensive and enlightening, Wound Healing: Methods and Protocols offers both basic and clinical scientists a thought-provoking and highly practical collection of widely used model systems and methods for studying the injury repair process.
 

What people are saying - Write a review

User Review - Flag as inappropriate

very informating book about wound healing

Contents

Excisional Wound Healing An Experimental Approach
3
Methods in Reepithelialization A Porcine Model of PartialThickness Wounds
17
Incisional Wound Healing Model and Analysis of Wound Breaking Strength
37
Animal Models of Ischemic Wound Healing Toward an Approximation of Human Chronic Cutaneous Ulcers in Rabbit and Rat
55
Corneal Injury A Relatively Pure Model of StromalEpithelial Interactions in Wound Healing
67
Subcutaneous Sponge Models
83
A Mouse Model of Burn Wounding and Sepsis
95
A Porcine Burn Model
107
Implantable Wound Healing Models and the Determination of Subcutaneous Collagen Deposition in Expanded Polytetrafluoroethylene Implants
263
The FibroblastPopulated Collagen Lattice A Model of Fibroblast Collagen Interactions in Repair
277
A Quantifiable In Vitro Model to Assess Effects of PAI1 Gene Targeting on Epithelial Cell Motility
293
Human Skin Organ Culture
305
In Vitro Matrigel Angiogenesis Model
311
Quantification of Wound Angiogenesis
319
In Vivo Matrigel Migration and Angiogenesis Assays
329
Endothelial Cell Migration Assay A Quantitative Assay for Prediction of In Vivo Biology
337

Wound Healing in Airways In Vivo
121
Murine Models of Intestinal Anastomoses
133
Murine Model of Peritoneal Adhesion Formation
141
Methods for Investigating Fetal Tissue Repair
149
Growth of Human Blood Vessels in Severe Combined Immunodeficient Mice A New In Vivo Model System of Angiogenesis
161
Tissue Repair in Models of Diabetes Mellitus A Review
181
Wound Healing Studies in Transgenic and Knockout Mice A Review
191
Wound Repair in Aging A Review
217
Specimen Collection and Analysis Burn Wounds
241
Suction Blister Model of Wound Healing
255
Analysis of Collagen Synthesis
349
Method for Detection and Quantitation of Leukocytes During Wound Healing
359
Detection of Reactive Oxygen Intermediate Production by Macrophages
369
Measurement of Chemokines at the Protein Level in Tissue
377
Methods of Measuring Oxygen in Wounds
389
Isolation Culture and Characterization of Human Intestinal Smooth Muscle Cells
417
Use of HighThroughput Microarray Membranes for cDNA Analysis of Cutaneous Wound Repair
425
ParticleMediated Gene Therapy of Wounds
433
Index
453
Copyright

Other editions - View all

Common terms and phrases

Bibliographic information