Handbook for Rhizobia: methods in legume-rhizobium technology
Rhizobia are bacteria which inhabit the roots of plants in the pea family and "fix" atmospheric nitrogen for plant growth. They are thus of enormous economic importance internationally and the subject of intense research interest. Handbook for Rhizobia is a monumental book of practical methods for working with these bacteria and their plant hosts. Topics include the general microbiological properties of rhizobia and their identification, their potential as symbionts, methods for inoculating rhizobia onto plants, and molecular genetics methods for Rhizobium in the laboratory. The book will be invaluable to Rhizobium scientists, soil microbiologists, field and laboratory researchers at agricultural research centers, agronomists, and crop scientists.
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General Microbiology of Rhizobia
Demonstrating Genetic Diversity in Rhizobia Using
Identification of Rhizobia
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acid agar agglutination ampoules antibiotic antibody antigen antisera antiserum Appendix approximately Autoclave bacteria bacteroids bags beaker blot bottle Bradyrhizobium Bradyrhizobium japonicum broth culture buffer carrier cells ml"1 centrifuge Chapter conjugate containing count deionized water dilution Dissolve distilled water enzyme Erlenmeyer flasks fermentor Figure filter paper fixation forceps freeze drying glass Gram Gram stain growth pouches Incubate inoculum isolates japonicum KEY REFERENCES Key Step KEY STEPS/OBJECTIVES legume Leonard jars Leucaena liter medium membrane method Microbiol microfuge tubes microtiter plate ml of sterile moisture NifTAL Nitrogen Fixation Pasteur pipette peat peat inoculant Petri dishes plant infection plasmid plastic Prepare probe reaction refrigerator replications rhizobia rhizobial strain Rhizobium Rhizobium spp saline samples seeds serological serum shaker sodium soil solution soybean species sterile water suspension symbiotic Table test tube Transfer chamber tray treatment volume wash water bath yeast-mannitol