Handbook for Rhizobia: Methods in Legume-Rhizobium Technology |
From inside the book
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Page 291
... microfuge tube holder out of a flat piece of polystyrene . Make holes in the polystyrene to fit the tubes snugly . This tube holder ( and float ) will be used later for holding the microfuge tubes during incubation in the water bath . Four ...
... microfuge tube holder out of a flat piece of polystyrene . Make holes in the polystyrene to fit the tubes snugly . This tube holder ( and float ) will be used later for holding the microfuge tubes during incubation in the water bath . Four ...
Page 308
... Microfuge tubes i . PEG Purification PEG , 13 % , w / v , in 1.6 M NaCl Microcentrifuge ( kept refrigerated ) , microfuge tubes Aspirator , water bath ( 48 ° C ) TE buffer , micropipettes , 0-1000 μl , and tips Phenol - chloroform ...
... Microfuge tubes i . PEG Purification PEG , 13 % , w / v , in 1.6 M NaCl Microcentrifuge ( kept refrigerated ) , microfuge tubes Aspirator , water bath ( 48 ° C ) TE buffer , micropipettes , 0-1000 μl , and tips Phenol - chloroform ...
Page 352
... microfuge tubes . Store in a freezer at -20 ° C . Medium Salt Buffer ( 10x ) 0.5 M Tris - HCl ( pH 8.0 ) 0.5 M NaCI 7.88 g Tris - HCl 2.9 g NaCl 0.1 M MgCl2 Water 0.95 MgCl2 100 ml Make up the solutions in distilled or deionized water ...
... microfuge tubes . Store in a freezer at -20 ° C . Medium Salt Buffer ( 10x ) 0.5 M Tris - HCl ( pH 8.0 ) 0.5 M NaCI 7.88 g Tris - HCl 2.9 g NaCl 0.1 M MgCl2 Water 0.95 MgCl2 100 ml Make up the solutions in distilled or deionized water ...
Contents
General Microbiology of Rhizobia | 1 |
Collecting Nodules and Isolating Rhizobia | 17 |
Observing the Infection Process | 24 |
Copyright | |
34 other sections not shown
Other editions - View all
Handbook for Rhizobia: Methods in Legume-Rhizobium Technology Padma Somasegaran,Heinz J. Hoben Limited preview - 2012 |
Handbook for Rhizobia: Methods in Legume-Rhizobium Technology Padma Somasegaran,Heinz J. Hoben No preview available - 2011 |
Handbook for Rhizobia: Methods in Legume-Rhizobium Technology Padma Somasegaran,Heinz J. Hoben No preview available - 1994 |
Common terms and phrases
acid agar agglutination ampoules antibiotic antibody antigen antisera antiserum Appendix approximately Autoclave bacteria bacteroids bags beaker blot bottle Bradyrhizobium Bradyrhizobium japonicum broth culture buffer carrier cells centrifuge Chapter containing count deionized water dilution Dissolve distilled water enzyme Erlenmeyer flasks fermentor Figure filter paper forceps freeze drying glass Gram stain growth pouches Incubate inoculum isolates japonicum Key Step KEY STEPS/OBJECTIVES legume leguminosarum bv Leonard jars Leucaena liter medium membrane method Microbiol microfuge tubes microtiter plate ml of sterile ml-¹ moisture NifTAL Nitrogen Fixation Pasteur pipette peat peat inoculant Petri dishes plant infection plasmid plastic Prepare probe reaction refrigerator replications rhizobia rhizobial strain Rhizobium Rhizobium spp Rinse root nodules saline samples seeds serological serum shaker sodium soil solution soybean species sterile water strains of rhizobia suspension symbiotic Table test tube Transfer chamber tray treatment volume wash water bath yeast-mannitol