Cytokines: A Practical Approach
Cytokines are important regulators of cellular communication, playing a central role in tissue development and repair, immunity and inflammation. They are also implicated in the pathophysiology of many infectious, autoimmune, and malignant diseases, and form the basis of novel therapeutic approaches. Their production is subject to complex and strict control, with local generation of a network of transiently induced cytokines.
Cytokines: A Practical Approach contains up-to-date methods for the study of cytokines, their receptors, and cytokine-driven processes, together with an overview of current cytokine biology. It provides a detailed research guide to the cloning, identification, characterization, and assay of cytokines, presenting key methods in molecular and cell biology, biochemistry, and immunology.
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R Contreras J Demolder and W Fiers
The generation and quantitation
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acid activity agarose aliquots analysis antigen assay binding Bioassay Biol biological blastocysts blot buffer cDNA cell lines cell suspension cell-line cells/ml centrifuge ceramide clones co-stimulation CO2 incubator concentration culture medium cytokine described in Protocol detection dilutions dishes EDTA electrophoresis enzyme Equipment and reagents factors feeder cells filter fragment gene GM-CSF growth medium holding medium human humidified hybridization IFN-y Immunol injection interferon labelled lipid lymphocytes lysates macrophages medium containing membrane methanol Method mg/ml microcentrifuge molecular monoclonal antibodies monocytes mouse mRNA murine NaCl ng/ml oocytes Pasteur pipette pellet peptide Pestka pg/ml pipette plasmid preparation probe production proliferation protein purified reaction receptor resuspend room temp RPMI samples Section sequences serum Sigma sodium solution Southern blot specific staining standard curve sterile stimulation supernatant target tissue culture transgenic Trypan blue tubes ug/ml virus Wash