Cytokines: A Practical ApproachFrances R. Balkwill Cytokines are important regulators of cellular communication, playing a central role in tissue development and repair, immunity and inflammation. They are also implicated in the pathophysiology of many infectious, autoimmune, and malignant diseases, and form the basis of novel therapeutic approaches. Their production is subject to complex and strict control, with local generation of a network of transiently induced cytokines. Cytokines: A Practical Approach contains up-to-date methods for the study of cytokines, their receptors, and cytokine-driven processes, together with an overview of current cytokine biology. It provides a detailed research guide to the cloning, identification, characterization, and assay of cytokines, presenting key methods in molecular and cell biology, biochemistry, and immunology. |
Contents
Cloning and expression of cytokine genes | 1 |
The generation and quantitation | 12 |
Receptorbinding studies | 17 |
41 other sections not shown
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acid activity agarose aliquots analysis antigen assay binding Bioassay Biol biological blot buffer cDNA cell lines cell suspension cell-line cells/ml centrifuge ceramide clones co-stimulation concentration culture medium cytokine described in Protocol detection digestion dilutions dishes EDTA ELISA enzyme Equipment and reagents ethanol ethidium bromide expression filter fragment gene GM-CSF growth medium holding medium human hybridization IFN-y Immunol Incubate injection interferon isolated labelled lipid lymphocytes lysates macrophages membrane Method mg/ml microcentrifuge microcentrifuge tube molecular monoclonal antibodies monocytes mouse mRNA murine NaCl ng/ml oocytes pellet peptide Pestka phospholipase pipette plasmid preparation probe procedure production protein purified radioactivity reaction receptor resuspend room temp RPMI samples scintillation Section sequences serum Sigma sodium solution Southern blot specific staining sterile stimulation supernatant target technique tissue culture transfer Trypan blue tubes vector virus vitro Wash µg/ml