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Acad Sci USA activity amino acid analysis antigen assay asymptomatic AzddU Bethesda binding CD4+ cells cell lines cellular CEM cells chimpanzees clinical clones coli concentrations culture cytotoxic detected determined dilution disease drug effect ELISA envelope glycoprotein epitope expression Gallo RC genome glycoprotein HIV infection HTLV HTLV-I HTLV-III human immunodeficiency virus immune response Immunol immunological incubated individuals induced infected cells inhibition isolates Laboratory lane lymphocytes macrophages Maryland medium mice molecular molecule monkeys monoclonal antibodies mononuclear mutant National Cancer Institute Natl Acad Sci p24 antigen PBMC peptide peripheral blood pol gene Proc Natl Acad protease protein purified reactivity receptor recombinant region replication retrovirus reverse transcriptase samples sCD4 SDS-PAGE sequence sera seropositive serum SIVAGM SIVMAC specific studies supernatant syndrome T-cell target tested titers transfected Tumor ug/ml vaccine vector viral virus infection virus type viruses vitro vivo Western blot Wong-Staal