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17D virus acute adult Aedes aegypti aleikii alimentary toxic aleukia animals antigen appear areas Asibi birds bite blood cage cent clinical comb complement-fixing Crimean hemorrhagic fever Culex dengue desiccated developed diagnosis dilution disease doses Eastern endemic epidemic hemorrhagic fever epidemiological equine encephalomyelitis EXPERIÊNCIA experiment experimental febrile feeding guinea pigs Hammon hemorrhagic fever horses human inactivation incubation period infected mosquitoes inoculated intracerebral intraperitoneally isolated Japanese B encephalitis July jungle yellow fever laboratory lesions liver lots Louis encephalitis Medical mice mosquitoes mouse brain nephroso-nephritis neutralizing antibodies normal observed obtained occurred Omsk hemorrhagic fever outbreak patients poliomyelitis Positive Positive Positive present protection test Public Health reaction reported rhesus septic angina showed species specimens spleen strain subcutaneously substrain survival susceptible suspension symptoms temperature ticks tion tissue titer titration transmission transmitted usually Uzbekistan vaccine vector viruses Western equine yellow fever virus