Basic Methods in Molecular Biology, Part 262
McGraw-Hill Professional Publishing, 1994 - Science - 777 pages
This edition emphasizes the decisions that need to be made to select one procedure over another in the everyday workings of a molecular biology laboratory. Trouble-shooting problems that may emerge when performing such methods are included and concise instructions are provided.
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Determining DNARNA Concentration
Bacterial Strains and Cloning Vectors
Enzymes that Modify DNA and RNA
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acrylamide adaptor agar agarose gel aliquot amplification annealing antibody aqueous phase assay autoclaving bacterial bacteriophage blot buffer cDNA library centrifugation chloroform clones colonies concentration containing cosmid denaturing digestion diluted DNA fragments DNA ligase dNTP EDTA efficiency eluted ethanol precipitation ethidium bromide exonuclease extraction filter first-strand cDNA fraction gel electrophoresis gene genomic DNA hybridization Incubate insert fragment kinase labeled ligase ligation lysate membrane METHODS In Advance mg/mL MgCl2 microcentrifuge tube mRNA NaCl nitrocellulose nucleic acid nucleotides oligonucleotide optimal pellet phage pipette plaques plasmid plasmid DNA plate polylinker polynucleotide Prepare probe procedure Promega protein protocol purified reaction REAGENTS recombinant remove restriction enzyme Resuspend RNase room temperature sample screening Section sequence single-stranded sodium acetate solution specific SS-phenol SS-phenol/chloroform Step strand Stratagene subcloning subtractive cDNA supernatant synthesis T4 DNA Taq DNA polymerase template termini tion tissue transcription transfection transfer U/uL volume wash