Basic Methods in Molecular Biology, Part 262This edition emphasizes the decisions that need to be made to select one procedure over another in the everyday workings of a molecular biology laboratory. Trouble-shooting problems that may emerge when performing such methods are included and concise instructions are provided. |
Contents
Introduction | 1 |
CL4B Chromatography | 36 |
Bacterial Strains and Cloning Vectors | 45 |
Copyright | |
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acrylamide adaptor agar agarose gel aliquot amplification annealing antibody aqueous phase autoclaving bacterial bacteriophage BamHI blot buffer cDNA library centrifugation chloroform clones colonies concentration containing cosmid denatured digestion diluted DNA fragments DNA ligase dNTP E coli EcoRI EDTA efficiency ethanol ethidium bromide exonuclease extraction filter first-strand cDNA gel electrophoresis gene genomic DNA H₂O hybridization Incubate kinase labeled ligase ligation membrane METHODS In Advance mg/mL MgCl2 microcentrifuge tube mRNA NaCl nitrocellulose nucleic acid nucleotides oligonucleotide pellet phage pipette plaques plasmid plasmid DNA plate polylinker polynucleotide precipitation Prepare primer probe procedure Promega protein protocol purified reaction REAGENTS recombinant remove restriction enzyme Resuspend RNase room temperature sample screening Section sequence single-stranded sodium acetate solution specific SS-phenol SS-phenol/chloroform Step sterile stored strand subcloning subtractive cDNA supernatant synthesis T4 DNA Taq DNA polymerase template termini tion tissue transcription transfection transfer U/µL wash