Molecular Cloning: A Laboratory Manual, Book 2 |
From inside the book
Results 1-3 of 96
Page 9-48
... probe added ( in micrograms ) , y = its complex- ity ( for most probes , complexity is proportional to the length of the probe in kilobases ) , and z = the volume of the reaction ( in milliliters ) . After hybridization to 3 × Cot1 / 2 ...
... probe added ( in micrograms ) , y = its complex- ity ( for most probes , complexity is proportional to the length of the probe in kilobases ) , and z = the volume of the reaction ( in milliliters ) . After hybridization to 3 × Cot1 / 2 ...
Page 9-53
... probe from binding to T - rich sequences that are found fairly commonly in eukaryotic DNA . 2. Float the ... probe is double - stranded , denature it by heating for 5 minutes at 100 ° C . Single - stranded probe need not be denatured ...
... probe from binding to T - rich sequences that are found fairly commonly in eukaryotic DNA . 2. Float the ... probe is double - stranded , denature it by heating for 5 minutes at 100 ° C . Single - stranded probe need not be denatured ...
Page 10-18
... probes are more stable than those formed between the probe and the target sequences . The resulting segregation of the probe leads to a decrease in the sensitivity with which the target sequences can be detected . The absence of a ...
... probes are more stable than those formed between the probe and the target sequences . The resulting segregation of the probe leads to a decrease in the sensitivity with which the target sequences can be detected . The absence of a ...
Other editions - View all
Common terms and phrases
acrylamide agarose gel aliquots amplification antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter chromatography cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing ddNTPs deletions denatured described digestion diluted dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers method MgCl2 microfuge tube minutes at 4°C molecules mRNA mutants Natl nitrocellulose nitrocellulose filters nucleic acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing reactions solution specific activity step stored strand of cDNA synthesis target DNA target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml