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Heterologous Expression of the Green Fluorescent Protein 78 1
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activity addition allow amount animal aperture applications assay beam bleaching buffer calcium camera Caution cell lines changes Chapter concentration construct containing contrast culture cytoplasm described determine diameter direct dish effect efficiency et al excitation experiments expression field FIGURE filter fluorescence focus FRAP gene glass green illumination important increase incubator injection intensity labeling laser lens light limited living measure medium membrane method micropipette microscope minutes objective observed oligonucleotide oocytes optical optimal phase plane plate position possible preparation probes protein protocol pulse range recovery region remove reporter resolution retroviral sample selection sequence signal solution specimen stage step sterile structure studies supernatant surface technique tion tissue transfection transfer tube typically usually vectors viral virus volume