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Monitoring Molecular Dynamics In Vivo Using Fluorescence Techniques 76
Heterologous Expression of the Green Fluorescent Protein 78 1
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acid adenovirus aequorin alignment amplicon animal antibody aperture assay beam Biol biological bleaching buffer calcium camera cationic lipid Caution cell lines cell types cellular Centrifuge Cepko Chapter cleotides clones concentration condenser confocal confocal microscope contrast coverslip culture cytoplasm diameter dish efficiency electroporation embryos ethanol excitation expression filter fluorophore FRAP gene transfer glass green fluorescent protein helper virus illumination incubator injection intensity labeling laser lens light lipid liposomes living cells medium membrane method microinjection micropipette microscope minutes molecular molecules monitoring objective oligonu oligonucleotide oocytes optical optimal packaging cell phase photobleaching photon pipette plane plasmid plate Prepare probes protocol pulse purification reagents recombinant recovery remove reporter gene Resuspend retroviral supernatants retroviral vector sample sequence signal solution specimen sterile supernatant syringe target cell technique tion tissue titer transfection tube typically viral vivo volume wavelength Xenopus