Cells: Light microscopy and cell structureCold Spring Harbor Laboratory Press, 1998 - Cytology Having identified a gene product, how do you determine what it does? The answer lies in Cells, a new manual designed to do for studies of cell biology what Cold Spring Harbor's Molecular Cloning has done for molecular biology.-- Sets the standard for techniques of proven bench reliability needed by all biomedical scientists studying cellular structure and function-- Delivers consistent, precisely crafted step-by-step protocols in an accessible format, with essential background details and in-depth advice on pitfalls and problem solving-- Created by three distinguished cell biologist/educators, from the contributions of over 180 leading cell biologists-- Complete with more than 300 expertly selected and superbly reproduced illustrations, over 70 in color. |
Contents
Monitoring Molecular Dynamics In Vivo Using Fluorescence Techniques 76 | 76-1 |
VOLUME 1 | 77-1 |
Heterologous Expression of the Green Fluorescent Protein 78 1 | 78-1 |
Copyright | |
101 other sections not shown
Other editions - View all
Common terms and phrases
acid adenovirus aequorin alignment amplicon animal antibody aperture assay beam Biol bleaching buffer calcium camera cationic lipid Caution cell lines cell types cellular Centrifuge Cepko Chapter cleotides clones concentration condenser confocal confocal microscope contrast coverslip culture cytoplasm diameter dish efficiency electroporation embryos ethanol expression filter fluorophore FRAP gene transfer glass green fluorescent protein helper virus illumination incubator injection intensity Köhler illumination labeling laser lens light lipid liposomes living cells medium membrane method mg/ml microinjection micropipette microscope minutes molecular molecules Natl objective oligonu oligonucleotide oocytes optical optimal packaging cell phase Photo photobleaching plane plasmid plate Prepare probes protocol pulse purification reagents recombinant recovery remove reporter gene Resuspend retroviral supernatants retroviral vector sample sequence signal solution specimen sterile supernatant syringe target cell tion tissue titer transfection tube viral vivo volume wavelength Xenopus µg/ml