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Monitoring Molecular Dynamics In Vivo Using Fluorescence Techniques 76
Heterologous Expression of the Green Fluorescent Protein 78 1
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activity addition allow amount animal applications assay beam Biol bleaching buffer calcium camera Caution cell lines changes Chapter concentration construct containing contrast culture cytoplasm described determine diameter direct dish effect efficiency et al example experiments expression field FIGURE filter fluorescence FRAP gene glass green HEPES important increase incubator indicator injection intensity labeling laser lens levels light living material measure medium membrane method microinjection micropipette microscope minutes monitoring NOTES objective oligonucleotide oocytes optical optimal phase photobleaching plane plate position possible Prepare probes protein protocol pulse range recovery region remove reporter retroviral sample selection sequence signal single solution specimen stage step sterile structure studies supernatant surface tion transfection transfer trap tube typically usually vectors viral virus volume