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autotrophic autotrophic bacteria bacteria Biochem biofilter Bremner Calvin cycle carbon assimilation cell free extract cell mass cell suspension cell yield chemolithotrophic chemolithotrophic bacteria chemostat culture citrate concentration of dimethyl continuous culture cytochrome dilution rate dimethyl sulphide dimethyl sulphide DMS disulphide DMS cells dry weight effluents electron energy enzymes extracts of Thiobaaillus facultative autotroph Figure filter following compounds formaldehyde glucose-6-phosphate glyoxylate growing on dimethyl grown on thiosulphate growth medium growth yield heterotrophic hydroxypyruvate reductase increase isocitrate lyase key enzymes l0 umol MATIN measured according metabolism methane methanol methyl mercaptan Methyloaoaaus aapsulatus methylotrophic methylotrophic bacteria micro-organisms Microbiol ml the cell NADH organic compounds oxaloacetate oxidation of thiosulphate oxygen consumption phosphoenolpyruvate protein Pseudomonas pyruvate reaction mixture containing reduced sulphur compounds reference cuvette lacked ribulose ribulose monophosphate route Rittenberg samples soil Specific activity substrate Table TCA cycle thio thiosulphate plus dimethyl thiosulphate-limited utilization vitamin volatile sulphur compounds ymol ZATMAN