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Escherichia coli Plasmids and Bacteriophages
Saccharomyces cerevisiae 131
Preparation and Analysis of DNA
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acetate acid activity adding addition agarose aliquots allow amount amplification Analysis antibody appropriate assay Basic Protocol bath beads buffer cDNA cells centrifuge cloning column complete concentration containing culture cycles database deionized water described determine digestion Dilute EDTA electrophoresis enzyme ethanol expression extract Figure filter final fragment fusion gene glass heat hybridization immediately Incubate Interactions kinase labeled medium membrane method mg/ml microcentrifuge tube Molecular Biology months NaCl overnight pellet peptide performed pipet Place plasmid plates polymerase precipitate Prepare primer probe protein Protocols in Molecular purification query reaction recombinant Remove Repeat Resuspend room temperature sample selection sequence Short Protocols slide sodium solution specific step sterile Store strain supernatant Support Protocol tissue transcription transfer transformation Tris-Cl tube ug/ml UNIT vector virus volume vortexing wash yeast