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activate transcription activation domain adenovirus affinity amino acid analysis assays basal transcription binding protein binding sites Biochemistry c-fos c-jun c-myc Cancer Research cDNA Cell Biology cell lines cell types cellular Center cerevisiae chromatin cloned Cold Spring Harbor conserved containing deletion Dept differentiation dimerization DNA binding activity DNA binding domain Drosophila encoding enhancer ETS1 eukaryotic function GAL4 GCN4 gene expression glucocorticoid heat shock heterodimer histone homeodomain hormone Hughes Medical Institute human identified induced interaction isolated kinase Laboratory mammalian mediated Medical School Molecular Biology motif mouse mRNA mutants N-terminal NF-kB nuclear extracts nucleosome octamer oncogene PEA3 phenotype phosphorylation preinitiation complex promoter purified receptor region repression repressor residues response element RNA polymerase RNAP RNAPII role sequence sequence-specific specific stimulate structure subunit TATA box template TFIIA TFIIB TFIID transactivation transcription factor transcription initiation transcriptional activation transcriptional regulation transfection upstream viral virus vitro transcription vivo yeast