Basic and Clinical Applications of Flow Cytometry: Proceeding of the 24th Annual Detroit Cancer Symposium Detroit, Michigan, USA - April 30, May 1 and 2, 1992
Frederick A. Valeriote, Alexander Nakeff, Manuel Valdivieso
Springer Science & Business Media, Dec 6, 2012 - Medical - 318 pages
The focus of this symposium was on the present and future capabilities of flow cytometry for both medical and biological applications in cancer. This technology began with quite modest instrumentation, with limited capabilities to answer biological questions. Today, both the clinical workhorses and the powerful multi-laser, multi-detector, sorting machinery, coupled with sophisticated computers and storage devices and the increasing storehouse of markers and dyes, are taking us to the limit and beyond in finding answers to the cause and cure of cancer.
In the past, both normal hematopoietic tissue and leukemias have been the tissue samples of choice in the application of flow cytometry, and some of the most recent applications with these tissues are presented here. However, the book also discusses the increasingly sophisticated disaggregation techniques which allow investigators the possibility to train their lasers on solid tumors. Not only can we use flow cytometry with associated fluorescent markers to understand the biology of cancer, but also the wide array of existing and developing markers provides us with important diagnostic tools in the detection of cancer early in either the malignant or relapse process. And the field comes full circle, with the use of the technology for gene mapping and other genetic studies to unlock the basic malignant process.
What people are saying - Write a review
We haven't found any reviews in the usual places.
MATERIALS AND METHODS
Other editions - View all
activation amplification anthracyclines antigen apoptosis Bcell bone marrow breast cancer canbe Cancer Res carcinoma CD4+ CD8+ Tlymphocytes cell cycle cell differentiation cell lines cell sorting cell subpopulations cellular chemotherapy chromosome clinical cmyc cyclins cytoplasmic cytotoxic debris detection diploid dissociation DNA analysis DNA aneuploid DNA content DNA diploid DNA flow cytometry DNA histograms DNA ploidy DNAcontent doxorubicin drug enzymatic enzyme expression factor fetal flow cytometric analysis flow cytometric DNA flow cytometry flow karyotype fluorescence fraction gene glutathione growth highspeed histograms human Immunol induced inhibition inthe intracellular leukemia lymphocytes lymphoma markers megakaryocytes method mitoxantrone molecular monoclonal antibodies multiparameter neuroblastoma node nuclear matrix nuclei ofcells ofDNA ofthe oncogenes Oncology osteosarcoma paraffinembedded parameters Pathol patients phenotype ploidy population principal component prochlorperazine prognostic protein rare cells RCS/Cy mice RCS5 mice receptor response samples SCCHN solid tumors specific Sphase staining stimulation studies techniques therapy tissue topoisomerase tumor tumor cells tumorcells vitro