Molecular Cloning: A Laboratory Manual, Book 3 |
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Results 1-3 of 76
Page 16-60
... reaction mixture 1 is required . 6. Mix each of the samples to be assayed with 80 μl of CAT reaction mixture 1 , and incubate the reactions at 37 ° C . The length of the incubation depends on the concentration of CAT in the cell extract ...
... reaction mixture 1 is required . 6. Mix each of the samples to be assayed with 80 μl of CAT reaction mixture 1 , and incubate the reactions at 37 ° C . The length of the incubation depends on the concentration of CAT in the cell extract ...
Page 16-63
... reaction , dilute the C - labeled acetyl coenzyme A tenfold into a solution of unlabeled acetyl coenzyme A ( 0.5 mM ) . The concentrations of the reagents in the final reaction mixture are : 2.3 mM chloramphenicol , 0.11 M Tris Cl , and ...
... reaction , dilute the C - labeled acetyl coenzyme A tenfold into a solution of unlabeled acetyl coenzyme A ( 0.5 mM ) . The concentrations of the reagents in the final reaction mixture are : 2.3 mM chloramphenicol , 0.11 M Tris Cl , and ...
Page 16-64
... reaction mixture is overlaid with water - immiscible scintillation fluid . Acetylated forms of chloramphenicol produced by the activity of CAT in the aqueous phase diffuse rapidly into the scintillation fluid ( Neumann et al . 1987 ) ...
... reaction mixture is overlaid with water - immiscible scintillation fluid . Acetylated forms of chloramphenicol produced by the activity of CAT in the aqueous phase diffuse rapidly into the scintillation fluid ( Neumann et al . 1987 ) ...
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Common terms and phrases
Acad acetate acrylamide activity agarose aliquots amino acids amount antibody antigen aqueous phase assay autoclaving autoradiographic bacteriophage T4 DNA blunt-ended bromophenol blue cDNA centrifugation chromatography codon coli DNA polymerase column containing culture Dissolve dithiothreitol DNA ligase DNA polymerase dNTPs double-stranded EDTA EDTA pH 8.0 efficiency eluted Escherichia coli ethanol ethidium bromide expression extract H H OH Incubate intensifying screen ligation linkers lysate mammalian cells medium method mg/ml microfuge tube mixture Molecular mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid nucleotides OH OH P.O. Box pasteur pipette pellet phenol phosphate pipette plasmid PMSF polyclonal precipitation prepared promoter radioactive radiolabeled reaction remove restriction enzyme room temperature sample Saran Wrap SDS gel-loading buffer SDS-polyacrylamide gel sequence single-stranded sodium sterile stock solution stored strain supernatant syringe target protein Telephone termini transcription transfected transfer Tris Cl pH vectors wash western blotting µg/ml